山东医药2017，Vol.57Issue(17)：10-12,16,4.DOI:10.3969/j.issn.1002-266X.2017.17.004
不同浓度依达拉奉对低氧环境下c-kit+心脏干细胞的影响
Effects of different concentrations of edaravone on c-kit+cardiac stem cells under hypoxic conditions
摘要
Abstract
Objective To observe the effects of different concentrations of edaravone on c-kit+cardiac stem cells(CSCs)under hypoxic conditions.Methods The freshly isolated hearts were perfused with collagenase buffer,and the hearts were dissected into single cells,c-kit+cells were purified from the cells by immunomagnetic beads.Flow cytometric analysis was used to measure the purity of c-kit+cells,immunofluorescence was used to analyze the phenotype.For further study,c-kit+CSCs were treated with different concentrations of edaravone(0,100,300,500,700,1 000 μmol/L),and then were divided into six groups.The reactive oxygen species(ROS)production was measured by DFCH-DA,apoptosis rate was analyzed by flow cytometer,cell migration was measured by 8-mm pore-size Transwell migration chamber,and the expression of basic fibroblast growth factor(bFGF)mRNA was determined by RT-PCR.Results The ROS production in the 1 000 μmol/L edavarone group was significantly decreased as compared with that of the other groups(all P<0.05).The apoptosis rate in the 1 000 μmol/L edavarone group was the lowest,and significant difference was found as compared with that in the 0 and 100 μmol/L edavarone groups(all P<0.05).The migration of c-kit+CSCs was the highest when edavarone concentration was 700 μmol/L,and significant difference was found as compared with that of the 0 μmol/L.When the concentration of edavarone was 700 μmol/L,the expression of bFGF mRNA in c-kit+CSCs was the highest(all P<0.05).Conclusion Edaravone has the protective effect on c-kit+CSCs by influencing the apoptosis rate,migration rate,ROS production and bFGF mRNA expression with a dose-dependent manner to some extent.关键词
心脏干细胞/依达拉奉/活性氧/细胞凋亡/细胞迁移/成纤维细胞生长因子Key words
cardiac stem cells/edaravone/reactive oxygen species/apoptosis/cell migration/fibroblast growth factors分类
医药卫生引用本文复制引用
文德重,周敏,薛松,黄日太..不同浓度依达拉奉对低氧环境下c-kit+心脏干细胞的影响[J].山东医药,2017,57(17):10-12,16,4.基金项目
上海市自然科学基金资助项目(134119a5602). （134119a5602）
