同济大学学报（医学版）2017，Vol.38Issue(2)：5-10,27,7.DOI:10.16118/j.1008-0392.2017.02.002
靶向TEM1蛋白促进细胞毒性药物Sap对TEM1高表达子宫肉瘤的杀伤作用
TEM1 antibody-conjugated Sap inhibits TEM1-positive human sarcoma cells in vitro and in vivo
摘要
Abstract
Objective To investigate the inhibitory effect of tumor endothelial marker 1 (TEM1) antibody-conjungated Sap on human sarcoma cells in vitro and in vivo.Methods Cytotoxic agent Sap was chemically conjugated to TEM1-antibody (anti-TEM1-Sap).The expression of TEM1 was detected by qPCR and flow cytomety in uterine sarcoma MES-SA,SKUT-1 cells,osteosarcoma HOS,SJSA-1,A673,U2OS,SAOS-2 cells,human fibrosacoma HT1080 cells and uterine carcinoma AN3 CA,RL95-2 and KLE cells.Human uterine sarcoma MES-SA cells were subcutaneous inoculated in nude mice.The rumor bearing mice were injected with anti-TEM1-Sap,and its inhibitory effect was analyzed.Results TEM was highly expressed in SJSA-1,A673,MES-SA and HOS cells.AntiTEM1-Sap at dose of 100 nmol/L significantly inhibited the rumor growth in uterine sarcoma-bearing nude mice.Conclusion TEM1-targeted immunotoxin can effectively inhibit TEM1-positive sarcoma in vitro and in vivo,which may have the potential for clinical application.关键词
肉瘤/肿瘤内皮标记物1/皂草毒蛋白/免疫毒性Key words
sarcoma/tumor endothelicial marker/Sap/immunotoxin分类
医药卫生引用本文复制引用
郭翼,郭飒,张颖,王建军,李怀芳,童晓文..靶向TEM1蛋白促进细胞毒性药物Sap对TEM1高表达子宫肉瘤的杀伤作用[J].同济大学学报（医学版）,2017,38(2):5-10,27,7.基金项目
国家自然科学基金(8110197) （8110197）
