同济大学学报（医学版）2017，Vol.38Issue(2)：33-38,6.DOI:10.16118/j.1008-0392.2017.02.007
microRNA-133a在复发性肝细胞癌中差异表达及其生物学功能研究
Differential expression and biological function of microRNA-133a in recurrent hepatocellular carcinoma
摘要
Abstract
Objective To investigate the expression of miR-133a in recurrent hepatocellular carcinoma and its relation to biological functions of tumor cells.Methods Differential expression of miRNA was screened in recurrent hepatocellular carcinoma by miRNA microarray.MiR-133a mimic or inhibitor was transfected into human hepatocellular carcinoma SMMC-7721 cells.Cell proliferation was detected by CCK8 assay and cell migration and invasion was examined by Transwell assay.The bioinformatics method was used to predict possible target genes of miR-133a.Results The expression of miR-133a was significantly increased in the early-recurrent HCC.There was no significant difference in cell proliferation activity between SMMC-7721 cells transfected with miR-133a mimic and those transfected with miR-133a inhibitor (P ＞ 0.05);however,the cell invasion and migration ability significantly enhanced (P ＜ 0.05) in SMMC-7721 cells after transfected with mimic,and decreased significantly after trasfected with miR-133a inhibitor (P ＜ 0.05).Conclusion The expressions of miR-133a is increased in hepatocellular carcinoma with early recurrence;miR-133a can promote the invasion and migration of rumor cells,and has no significant effect on cell proliferation,suggesting that elevated miR-133a levels may increase the risk of intrahepatic metastasis and recurrence of hepatocellular carcinoma.关键词
miR-133a/肝细胞癌/生物学功能Key words
miR-133a/hepatocellular carcinoma/biological function分类
医药卫生引用本文复制引用
陆新元,谷晓媛,纪义梅,赵骞,盛霞,丛文铭..microRNA-133a在复发性肝细胞癌中差异表达及其生物学功能研究[J].同济大学学报（医学版）,2017,38(2):33-38,6.基金项目
国家自然基金青年项目(81602603) （81602603）
国家自然基金面上项目(81272662) （81272662）
上海市卫生和计划生育委员会青年科研项目(2013Y121、20144Y0047) （2013Y121、20144Y0047）
