同济大学学报（医学版）2017，Vol.38Issue(2)：39-44,6.DOI:10.16118/j.1008-0392.2017.02.008
抗SMO多克隆抗体对DU145前列腺癌细胞生长及侵袭力的影响
Effects of SMO polyclonal antibody on proliferation and invasive ability of prostate cancer DU145 cells
摘要
Abstract
Objective To investigate the effects of SMO polyclonal antibody on proliferation and invasive ability of human prostate cancer DU145 cells.Methods SMO protein epitope was predicted and used for preparation of polyclonal antibody.Titer and specificity of antibody was evaluated.DU145 cells were divided into blank control group,normal rabbit IgG group,SMO antibody group,shRNA vector control group,TRPC-6 shRNA group and combined normal IgG and SMO antibody group.Cell proliferation and apoptosis was detected by MTT assay and flow cytometry,respectively;and cell invasive ability was examined by Transwell assay.Results Titer of antibody in this study was 1∶51 200.The antibody was verified to be specific.MTT assay indicated that the proliferation of DU145 cells was inhibited with the increasing of SMO antibody concentrations (P ＜ 0.05).Flow cytometry showed that the ratio of apoptotic cells increased in SMO antibody group compared to normal IgG group (P ＜ 0.05);Transwell assay showed that invasive ability was decreased by SMO antibody.In TRPC-6shRNA stable transfected DU145 cells,invasive inhibition rate was increased (P ＜ 0.05).Conclusion SMO polyclonal antibody can inhibit proliferation and enhance apoptosis of prostate cancer DU145 cells,and it may have a synergistic effect with TRPC-6shRNA.关键词
多克隆抗体/DU145/前列腺肿瘤/SMO/TRPC-6/兔Key words
polyclonal antibody/DU145/prostate cancer/SMO/TRPC-6/rabbit分类
医药卫生引用本文复制引用
杨波,温晓飞,刘辉,刘峰,邓晓俊,廖国强..抗SMO多克隆抗体对DU145前列腺癌细胞生长及侵袭力的影响[J].同济大学学报（医学版）,2017,38(2):39-44,6.基金项目
中国临床医学诊疗技术创新发展专项基金(320.6750.14197) （320.6750.14197）
