陕西师范大学学报（自然科学版）2017，Vol.45Issue(3)：112-118,7.DOI:10.15983/j.cnki.jsnu.2017.03.234
间歇运动上调CTRP3表达抑制心梗大鼠心肌细胞凋亡及其机制
Interval exercise training up-regulates CTRP3 expression and inhibits cardiomyocyte apoptosis in rat with myocardial infarction
摘要
Abstract
This study was carried out to detect the effects of interval exercise training on cardiomyocyte apoptosis and the expression of C1q/tumor necrosis factor-related protein-3(CTRP3) in rat with myocardial infarction(MI).Adult male sprague-dawley rats, 3-mouth old, were randomly divided into three groups: Sham-operated group (Sham), sedentary MI group (MI) and MI with interval exercise group (ME).The MI model was established by ligation of the left anterior descending coronary artery, and rats in ME were subjected to 8 weeks of interval exercise training.Cardiac function, heart tissue remodeling and cardiomyocyte apoptosis were evaluated after the training.The expression of CTRP3, p-AKT, AKT, p-mTOR, mTOR, Cytochrome C, Bcl-2, Bax and BNP was determined in the peri-infarcted area of the left ventricle by Western Blot or RT-qPCR.MI resulted in cardiac dysfunction and myocardial fibrosis, increased the mRNA expression of CTRP3 and reduced its protein expression, activated the phosphorylation of Akt and mTOR, induced cardiomyocyte apoptosis and up-regulated the BNP protein expression.Compared with MI group, interval exercise training significantly improved cardiac function,attenuated myocardial remodeling by reducing CVF%, up-regulated the CTRP3 expression and phosphorylation of Akt and mTOR,and inhibited cardiomyocyte apoptosis in MI rats.Interval exercise training up-regulated CTRP3 expression,activated Akt/mTOR signaling in infarcted heart, inhibited cardiomyocyte apoptosis, and improved cardiac function.关键词
间歇运动/心肌梗死/细胞凋亡/CTRP3Key words
interval exercise training/myocardial infarction/cardiomyocyte apoptosis/CTRP3分类
社会科学引用本文复制引用
候璐,伏睿臻,席悦,田振军..间歇运动上调CTRP3表达抑制心梗大鼠心肌细胞凋亡及其机制[J].陕西师范大学学报（自然科学版）,2017,45(3):112-118,7.基金项目
国家自然科学基金(31371199,31171141) （31371199,31171141）
