山西医科大学学报2017，Vol.48Issue(5)：409-414,6.DOI:10.13753/j.issn.1007-6611.2017.05.002
盐酸千金藤碱对HepG2.2.15细胞HBX/NF-κB通路的影响
Effect of cepharanthine hydrochloride on HBX/NF-κB signal pathway of HepG2.2.15 cells
张晶敏 1魏青青 2马琳 1吕晓瑞 1江金花 1王庆端1
作者信息
- 1. 河南省医药科学研究院,河南省肝病药理重点实验室,郑州450052
- 2. 郑州大学第一附属医院药学部
- 折叠
摘要
Abstract
Objective To investigate the effect of cepharanthine hydrochloride(CH) on HBX/NF-κB signal pathway of HepG2.2.15 cells stably expressing wide-type HBV.Methods HepG2.2.15 cells were treated with different concentration of CH (2.5,5,10 μmol/L).Then the expression levels of X gene and X protein of HBV weredetected by reverse thanscriptase polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay(ELISA),respectively.Flow cytometry method was used to detect the cell apoptosis rates.The expression levels of IκBα and P65 protein in HepG2.2.15 cells were detected by Western blot.Results Compared with HepG2.2.15 cells without CH treament(control group),the expression of X gene mRNA and X protein in HepG2.2.15 cells after treatment with CH(2.5,5,10 μmol/L) were significantly inhibited(P ＜ 0.05).The apoptosis retes of HepG2.2.15 cells were (11.9 ± 1.21) ％,(19.8 ± 2.32) ％,(29.3 ± 1.27) ％ after treatment with 2.5,5,10 μmol/L CH for 48 h,respectively.CH increased apop-tosis rates of HepG2.2.15 cells in a concentration-dependent manner.CH effectively up-regulated the protein expression of IκBα and down-regulated the expression of P65 protein (both P ＜ 0.05).Conclusion CH can inhibit the activity of HBx/NF-κB signal pathway and increase cell apoptosis of HepG2.2.15 cells.关键词
盐酸千金藤碱/乙型肝炎病毒/HBV X蛋白/核转录因子-κB/HepG2.2.15细胞Key words
cepharanthine hydrochloride/hepatitis B virus/HBx/NF-κB/HepG2.2.15 cells分类
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张晶敏,魏青青,马琳,吕晓瑞,江金花,王庆端..盐酸千金藤碱对HepG2.2.15细胞HBX/NF-κB通路的影响[J].山西医科大学学报,2017,48(5):409-414,6.
