水土保持学报2017,Vol.31Issue(2):166-170,203,6.DOI:10.13870/j.cnki.stbcxb.2017.02.028
溶磷细菌对复垦土壤养分、酶活性及磷解析的影响
Effect of Phosphobacterias on Nutrient, Enzyme Activities and Phosphorus Adsorption-Desorption Characteristics in a Reclaimed Soil
摘要
Abstract
The aim of this paper was to study the effect of phosphobacterias on phosphorus in a reclaimed soil.The tricalcium phosphate-solubilizing ability of individual phosphobacteria and their combinations was tested using laboratory shake-flask culture.The culture broth of Rahnella sp (W2) + Fluorescent pseudomonas1 (W3)+ Fluorescent pseudomonas2 (W4) produced the highest available P content,609.1 mg/L.Therefore,W2+W3+W4 was selected as the optimal combination of phosphobacterias.The effects of the optimal combination of phosphobacterias on soil nutrients,enzyme activities,P desorption properties,and maize yield were investigated by carrying out a field experiment in a reclaimed soil in a coal-mining subsidence area.The results showed that phosphobacterias increased soil nutrient contents and improved soil enzyme activities.Soil available P and K contents,as well as phosphatase,invertase,and urease activities,were increased by 2.8,25.43,7.41,4.21 and 7.15 mg/kg,respectively,by phosphobacterias compared to the medium control.The greatest reduction in the maximum P adsorption capacity (114 mg/kg) and adsorption constant (0.021) of soil was found with phosphobacterias compared to the blank control (p<0.05).The mean P desorption rate of soil with phosphobacterias was 14.7,significantly higher than those in other treatments.The highest maize yield was also obtained with phosphobacterias,which was 8 036 kg/hm2 and significantly different from those in other treatments.In conclusion,the application of phosphobacterias could improve soil P desorption properties,facilitate rapid improvement of soil fertility,and contribute to crop yield in reclaimed soils.关键词
最佳组合磷细菌/复垦土壤/土壤养分/磷解析特性Key words
the best combination of phosphobacteria/reclaimed soils/soil nutrient/phosphorus adsorption-desorption characteristics分类
农业科技引用本文复制引用
乔志伟,洪坚平,李林轩,刘超..溶磷细菌对复垦土壤养分、酶活性及磷解析的影响[J].水土保持学报,2017,31(2):166-170,203,6.基金项目
国家自然科学基金项目(31272257) (31272257)
贵州省科技厅、安顺市人民政府、安顺学院三方联合基金项目黔科合LH字(2016-7280号) (2016-7280号)