西北师范大学学报（自然科学版）2017，Vol.53Issue(3)：88-92,105,6.DOI:10.16783/j.cnki.nwnuz.2017.03.016
河西走廊盐碱土中未培养放线菌多样性研究
Research on diversity of uncultured actinomycetesin saline-alkali soil from Hexi Corridor
摘要
Abstract
To amplify 16S rRNA gene fragments actinomycetes using specific primers,and construct actinomycetes 16S rRNA clone library,the purpose of this study is to explore the saline-alkali soil actinomycetes species group structure and diversity from Hexi Corridor.The results show that 164 positive clones points attribute to 50 OTUs,phylogenetic analysis show that 50 OTUs belong Propionibacterineae,Pseudonocardineae,Corynebacterineae,Micromonosporineae,Streptomycineae,Micrococcineae,and Streptosporangineae actinomycetes.Micrococcineae is the dominant populations which account for 37.2% in the clone library.In addition,there is an unknown populations of clones belonging to the Actinobacteria,which account for 14.01%.The dilution curves of saline-soil is not level off,which indicate the actinomycetes diversity in saline-soil is more abundant.关键词
盐碱土壤/放线菌/克隆文库/多样性Key words
saline-alkali soil/actinomycetes/clone library/diversity分类
生物科学引用本文复制引用
牛世全,龙洋,李海云,李渭娟,胡山..河西走廊盐碱土中未培养放线菌多样性研究[J].西北师范大学学报（自然科学版）,2017,53(3):88-92,105,6.基金项目
国家自然科学基金资助项目(31260134) （31260134）
