中国临床药理学与治疗学2017，Vol.22Issue(4)：361-366,6.
脂氧素A4抑制缺氧诱导的滋养细胞氧化应激和凋亡
Lipoxin A4 attenuates hypoxia induced oxidative stress and apoptosis in human first trimester trophoblast cells
摘要
Abstract
AIM:To investigate the effect of lipoxin A4 (LXA4) on oxidative stress and apoptosis of human first trophoblast cells induced by hypoxia.METHODS:Primary human trophoblast cells were randomized into normoxia group,hypoxia group and hypoxia + LXA4 (1,10 and 100 nmol/L) groups.The level of ROS was determined by DCFH-DA,the activity of SOD,CAT and GPx were detected by the corresponding kits.The apoptosis rate was analyzed by flow cytometer method and the level of Bcl-2 and Bax protein were observed by Western Blot.RESULTS:Compared with normoxia group,the level of ROS and apoptosis rate increased (P ＜ 0.05) while the activity of SOD,CAT and GPx decreased (P ＜0.05) in hypoxia group.Compared with hypoxia group,the level of ROS and apoptosis rate decreased (P ＜ 0.05),while the activity of SOD,CAT and GPx increased (P ＜ 0.05) in hypoxia + LXA4 (1,10and 100 nmol/L) groups.Compared with normoxia group,the level of Bcl-2 protein in hypoxia group decreased and the level of Bax protein increased (P ＜ 0.05).Compared with hypoxia group,the level of Bcl-2 protein in hypoxia + LXA4 (1,10 and 100 nmol/L) groups increased and the level of Bax protein decreased (P ＜ 0.05).CONCLUSION:Lipoxin A4 attenuates hypoxia induced oxidative stress and apoptosis in human first trophoblast cells.The regulation of Bcl-2/Bax expression may be an important mechanism of LXA4 in inhibiting apoptosis.关键词
脂氧素/滋养细胞/缺氧/氧化应激/凋亡Key words
lipoxin/trophoblast/hypoxia/oxidative stress/apoptosis分类
医药卫生引用本文复制引用
黄艳君,吴洁,蒋维,王晓,黄引平..脂氧素A4抑制缺氧诱导的滋养细胞氧化应激和凋亡[J].中国临床药理学与治疗学,2017,22(4):361-366,6.基金项目
国家自然科学基金项目(81070510、81671480) （81070510、81671480）
浙江省自然科学基金项目(Y2101233、LY16H040009) （Y2101233、LY16H040009）
