中国临床药理学与治疗学2017，Vol.22Issue(4)：367-372,6.
黄蜀葵花总黄酮对海马神经元缺氧复氧损伤的保护作用
Protective effect of total flavones of abelmoschl manihot on hippocampal neurons subjected to anoxia-reoxygenation injury
摘要
Abstract
AIM:To study the protective effect of total flavones of abelmoschl manihot (TFA) on hippocampal neurons subjected to the injury of anoxia/re-oxygenation (A/R) and its endothelium-dependent hyperpolarizing factor (EDHF) mechanism.METHODS:The injury of primarily cultured hippocampal neurons plus with cerebral basilar artery ring was induced by hypoxia for 4 hours and re-oxygenation for 24 hours,pretreated with TFA,acetylcholine (Ach),etc.Cell viability and the lactate dehydrogenase (LDH) activity in supematant were served as the cell damage index.The level of free calcium fluorescence intensity in the neurons was detected by laser scanning confocal microscope.RESULTS:Pretreated with TFA and cerebral artery could increase the cell viability,reduce the activity of LDH in culture medium significantly,pretreated with Ach had a similiar effect.The N-nitro-L-arginine-methyl-ester (L-NAME) and indomethacin (Indo) didn't influence the effect of Ach or TFA.Moreover,pretreated with TFA plus with cerebral artery lowered the calcium concentration in neuron (compared with model group).CONCLUSION:TFA has remarkably neuroprotective effect induced by A/R,the mechanism is partially concerned with the calcium overload inhibited by EDHF released from cerebral basilar artery ring.关键词
黄蜀葵花总黄酮/内皮衍生超级化因子/缺氧/复氧损伤/乳酸脱氢酶/钙超载Key words
TFA/EDHF/A/R injury/LDH/calcium concentration分类
医药卫生引用本文复制引用
文继月,陈志武..黄蜀葵花总黄酮对海马神经元缺氧复氧损伤的保护作用[J].中国临床药理学与治疗学,2017,22(4):367-372,6.基金项目
国家自然科学基金(81173596) （81173596）
