中国临床药理学与治疗学2017，Vol.22Issue(4)：373-380,8.
淫羊藿素通过抑制Hedgehog信号通路保护终板软骨细胞退变
Icaritin protects the ICMT-induced degeneration of endplate chondrocytes via inhibiting Hedgehog signaling pathway
摘要
Abstract
AIM:To investigate the effect of icaritin on degeneration of endplate chondrocytes induced by intermittent cyclic mechanical tension (ICMT) and its mechanism in vitro.METHODS:Rat endplate chondrocytes degeneration model was established by using FX-5000T flexcell stress loading system in vitro.Three groups were erected:normal control group (NC group),ICMT group (10％ intermittent cyclic mechanical tension,10％ ICMT,0.5 Hz,8 h/d),and ICT + ICMT group.The morphological alteration of the cells was observed with toluidine blue staining and phalloidin staining.Cell proliferation and apoptosis were detected respectively by CCK-8 and flow cytometry.The mRNA levels of chondrocyte-associated genes were detected by qRTPCR and protein levels were determined by Western blot.RESULTS:Degeneration of endplate chondrocytes was apparently observed after mechanical stimulation.In detail,cell morphology changed from polygon to long spindle after mechanical loading.The cell proliferation rates in ICMT group and ICT +ICMT group were higher than NC group.However,there was no significant difference between ICMT group and ICT + ICMT group.The apoptosis rate of ICT + ICMT group was significantly lower than ICMT group.Interestingly,treatment with icariin effectively alleviated ICMT-induced degeneration of endplate chondrocytes,and significantly suppressed the Hedgehog signaling pathway.CONCLUSION:Icaritin can efficiently protect the ICMT-induced cartilage degeneration by inhibiting the activity of Hedgehog signaling pathway.关键词
淫羊藿素/终板软骨细胞/细胞退变/Hedgehog信号通路Key words
icaritin/endplate chondrocytes/cell degeneration/Hedgehog signaling pathway分类
医药卫生引用本文复制引用
徐永明,徐宏光,高智,张晓玲..淫羊藿素通过抑制Hedgehog信号通路保护终板软骨细胞退变[J].中国临床药理学与治疗学,2017,22(4):373-380,8.基金项目
国家自然科学基金(81272048) （81272048）
卫生部公益性行业专项基金(201002018) （201002018）
