中国临床药理学与治疗学2017，Vol.22Issue(4)：381-386,6.
MiR-200a抑制Wnt/β-catenin信号通路缓解肾间质纤维化
MiR-200a alleviates renal interstitial fibrosis by inhibiting Wnt/β-catenin signaling pathway
摘要
Abstract
AIM:To investigate the inhibition of miR-200a on Wnt/β-catenin signaling pathway in unilateral ureteral obstruction mouse,and the effects of miR-200a on renal interstitial fibrosis.METHODS:Models of renal interstitial fibrosis were established by unilateral ureteral obstruction (UUO).Twenty-four six-weeks C57BL/6 male mice were randomly divided into four groups (n =6),Sham group,UUO group,UUO + miR-200a group and UUO + NC group.From day 1,UUO + miR-200a group was given miR-200a (i.v.by tail vein,40mg/kg),while UUO + NC group was given Negative Control (i.v.by tail vein,40 mg/kg).Both groups were administered for three consecutive days.On day 7,HE and Masson staining were used to detect pathological changes in kidneys;RT-PCR and Western blot were used to detect mRNA and protein expressions of Wnt4,β-catenin,Fibronectin and α-SMA.RESULTS:Compared with Sham group,UUO group exhibited obvious renal tubular damage and interstitial collagen deposition;expressions of Wnt4,β-catenin,Fibronectin and α-SMA mRNAs and proteins were also increased in UUO group (P ＜0.05).Compared with UUO group,UUO + miR-200a group presented minor renal tubular injury and interstitial collagen deposition;expressions of Wnt4,β-catenin,Fibronectin α-SMA mRNAs and proteins were also reduced in UUO + miR-200a group (P ＜0.05).CONCLUSION:miR-200a can alleviate renal interstitial fibrosis by inhibiting the Wnt/β-catenin signaling pathway.关键词
miR-200a/Wnt/β-catenin信号通路/肾间质纤维化Key words
miR-200a/Wnt/β-catenin signaling pathway/renal interstitial fibrosis分类
医药卫生引用本文复制引用
陈华,龚艺,周宝尚,张璟..MiR-200a抑制Wnt/β-catenin信号通路缓解肾间质纤维化[J].中国临床药理学与治疗学,2017,22(4):381-386,6.基金项目
国家自然科学基金项目(81270775) （81270775）
