中国临床药理学与治疗学2017，Vol.22Issue(4)：387-393,7.
中药玉夏胶囊对自发性高血压大鼠肾纤维化的改善作用及TGF-β1/Smads信号通路的影响
Effect of traditional Chinese medicine Yuxia capsule on renal fibrosis in spontaneously hypertensive rats and its relationship with TGF-β/Smads signaling pathway
摘要
Abstract
AIM:To observe the effect of traditional Chinese medicine Yuxia capsule (YXC) on renal fibrosis in spontaneously hypertensive rats (SHR) and its relationship with TGF-β1/Smads signaling pathway.METHODS:Twenty-eight 14-week-old male SHR were randomized into four groups (n=7),i.e.SHR group (0.5％ CMC-Na,5 mL/kg),YXC high dose group (0.6 g/kg),middle dose group (0.3 g/kg) and low dose group (0.15 g/kg).Another 7 wistar-kyoto(WKY) rats were selected as normal group (0.5％ CMC-Na,5mL/kg).Animals in each group were intragastrically administrated with YXC once a day for consecutive 10 weeks.Blood pressure was measured before administration and every two weeks during administration by tail cuff method.After the last administration 24 h urine was collected and the urine micro albumin (U-mALb) levels were detected.Serum levels of beta 2-microsphere protein (β2-MG),plasma and renal tissue angiotensin Ⅱ (AngⅡ) were measured.Changes of renal histology and collagen fibers were observed under HE and Masson staining.Expressions of TGF-β1,Smad4,Smad7 protein and Smad2/3 protein phosphorylation were detected in renal tissues by Western blot.RESULTS:Compared with model group,YXC significantly decreased the blood pressure of SHR,levels of U-mALb,β2-MG,plasma and renal tissue AngⅡ (P ＜ 0.01 or P ＜0.05),and it significantly ameliorated the renal pathological tissue damage and the collagen fiber deposition.Expressions of TGF-β1,Smad4 proteins and phosphorylation of Smad2/3 protein level in YXC group were lower than those in model group,while Smad7 protein expression was higher (P ＜0.01).CONCLUSION:Traditional Chinese medicine YXC demonstrated protective effects against renal injury in a dose-dependent manner within a certain range in SHR,of which the potential mechanism may be related to decreasing the blood pressure,down-regulating AngⅡ level and inhibiting the excessive activation of TGF-β1/Smads signaling pathway.关键词
玉夏胶囊/自发性高血压大鼠/肾纤维化/血管紧张素Ⅱ/TGF-β1/Smads信号通路Key words
Yuxia capsule/spontaneously hypertensive rats/renal fibrosis/AngⅡ/TGF-β1/Smads signaling pathway分类
医药卫生引用本文复制引用
胡坤媚,胡浩然,沈媛媛,卢毅宁,杨解人,韩军..中药玉夏胶囊对自发性高血压大鼠肾纤维化的改善作用及TGF-β1/Smads信号通路的影响[J].中国临床药理学与治疗学,2017,22(4):387-393,7.基金项目
安徽省教育厅自然科学基金重点项目(KJ2015A157) （KJ2015A157）
