中国临床药理学与治疗学2017，Vol.22Issue(4)：406-411,6.
蛋白酶体抑制剂减少病毒性心肌炎小鼠炎症因子IL-6和TNF-α的表达
Proteasome inhibitors decrease IL-6 and TNF-α levels in mouse acute viral myocarditis
摘要
Abstract
AIM:To investigate the effect of proteasome inhibitor in mice with inflammatory reaction of acute viral myocarditis induced by coxsackievirus B3 virus (CVB3) infection.METHODS:100 male BALB/C mice were randomly divided into four groups,i.e.the normal control group,CVB3 group,CVB3 and bortezomib group,CVB3 and MG-132 group.Mice in control group were injected with blank solvent,while mice in other three groups were intraperitoneally inoculated with CVB3 to induce acute viral myocarditis.24 h after infection,mice in treatment groups were administrated with bortezomib or MG-132 respectively for 7d continuously by intraperitoneal injection.Changes of myocardial ultrastructure,the mRNA and protein levels of IL-6 and TNF-α,the number of PMN,and the survival rate of each group were detected.RESULTS:Compared with control group,the protein levels of IL-6 and TNF-α,and the number of polymorphonuclear neutrophils (PMN) were significantly increased in CVB3 group (P ＜ 0.05).The cardiomyocytes were diffused and swelled,the myofilament was lysed,and the mitochondria was swelled and vacuolizated.The above mentioned inflammatory factors in bortezomib group and MG-132 group were significantly decreased as compared with CVB3 group (P ＜0.05).The damaged degree of the cardiomyocytes in treatment groups were less,and the mortality due to deadly arrhythmia were lower than that in CVB3 group.CONCLUSION:Proteasome inhibitors protect the mice from CVB3-induced acute viral myocarditis by suppressing the expression of inflammatory factors.Ubiquitin proteasome system is a potential new target therapy for viral myocarditis.关键词
病毒性心肌炎/MG-132/炎症因子/嗜中性多型核白细胞/IL-6/TNF-αKey words
viral myocarditis/MG-132/inflammatory factor/PMN/IL-6/TNF-α分类
医药卫生引用本文复制引用
张新民,陈鹏,叶盛,夏武杰,李岳春..蛋白酶体抑制剂减少病毒性心肌炎小鼠炎症因子IL-6和TNF-α的表达[J].中国临床药理学与治疗学,2017,22(4):406-411,6.基金项目
国家自然科学基金项目(81570342) （81570342）
温州市科技局科技计划项目(Y20130178) （Y20130178）
