中国临床药理学与治疗学2017，Vol.22Issue(4)：412-417,6.
泛素特异性肽酶22诱导胰腺癌细胞对吉西他滨耐药的观察
Effect of USP22 on gemcitabine chemoresistance in human pancreatic cancer cell
王宛明 1董贾中 1管淑敏1
作者信息
- 1. 河南省濮阳市人民医院消化科,濮阳457000,河南
- 折叠
摘要
Abstract
AIM:To evaluate the potential effect of USP22 on gemcitabine chemoresistance in pancreatic cancer cells.METHODS:USP22 expression was detected by Western blot in pancreatic cancer cell lines PANC-1 and SW1990 treated with gemcitabine.The IC50 values of different pancreatic cancer cells to gemcitabine were detected by cell counting kit 8 (CCK-8) assays.RESULTS:USP22 expression was induced by certain dose of gemcitabine treatment in pancreatic cancer cells.CCK-8 assay demonstrated that the IC50 values in SW1990NC group and SW1990-shUSP22 group were (1 850.96 ± 87.37) nmoL/L and (534.83 ± 49.68) nmol/L,respectively.Furthermore,the IC50 values in SW1990-NC,SW1990/Gem-NC and SW1990/Gem-shUSP22 groups were (1 387.58 ±96.56) nmol/L,(29 850.96 ± 345.78) nmol/L and (2 157.08 ± 120.32) nmol/L,respectively.CONCLUSION:Gemcitabine treatment increases the expression of USP22 in pancreatic cancer cells.Knock-down USP22 expression could enhance the chemosensitivity to gemcitabine in pancreatic cancer cells,but also rescue the gemcitabine resistance in pancreatic cancer cells.关键词
泛素特异性肽酶22/胰腺癌/吉西他滨/耐药Key words
USP22/pancreatic cancer/gemcitabine/resistance分类
医药卫生引用本文复制引用
王宛明,董贾中,管淑敏..泛素特异性肽酶22诱导胰腺癌细胞对吉西他滨耐药的观察[J].中国临床药理学与治疗学,2017,22(4):412-417,6.
