中国临床药理学与治疗学2017，Vol.22Issue(4)：418-423,6.
百事乐胶囊对抑郁模型大鼠海马隔颞轴BDNF、NT-3、NGF表达的影响
Effect of Baishile capsule on expressions of BDNF, NT-3, NGF in septo-temporal axis of hippocampus of depressive rats
摘要
Abstract
AIM:To investigate the effect of Baishile capsule on the expression of neurotrophic factor including brain-derived neurotrophic factor (BDNF),neurotrophin 3 (NT-3),and nerve growth factor (NGF) in hippocampal septo-temporal axis of depressive rats.METHODS:Sixty SD rats were randomly divided into control group,model group of depression,fluoxetine group,high (2.88 g/kg),medium (1.44 g/kg) and low (0.72 g/kg) doses of Baishile capsule.Chronic unpredictable mild stress for 21 d was used to establish depression model;sucrose preferences and forced swimming test to detect depression-like behavior in rats;Morris water maze test to evaluate learning and memory ability;immunofluorescence technique to observe the content change of neurotrophic factors (BDNF,NT-3,NGF) in hippocampal septo-temporal axis of rats.RESULTS:Compared with the control group,sucrose preference was decreased while immobility time was increased significantly (P ＜ 0.01);escape latency and latency time in target quadrant presented a significant increase (P ＜ 0.01),;times of crossing the target quadrant were significantly reduced (P ＜ 0.05);BDNF,NT-3,NGF expressions were down-regulated in S1,S2,T3 and T4 parts of hippocampal septo-temporal axis in model rats (P ＜0.01).Compared with model group,Baishile capsule could significantly improve sucrose preference degree and decrease immobility time (P ＜ 0.05);escape latency and latency time in target quadrant were significantly shorter (P ＜ 0.05);times of crossing the target quadrant increased in rats after administration of Baishile capsule (P ＜ 0.05);BDNF,NT-3,NGF expressions increased in S2 and T3 parts of septo-temporal axis in depressive rats (P ＜0.05 or P ＜ 0.01).CONCLUSION:Baishile capsule could significantly improve the depression-like behavior,learning and memory ability in depressive rats,which might be associated with regulating the function of S2,T3 parts of hippocampal septo-temporal axis,and promoting the expression of neurotrophic factors.关键词
百事乐胶囊/抑郁/慢性应激/海马/隔颞轴/神经营养因子Key words
Baishile capsule/depression/chronic stress/hippocampus/septo-temporal axis/neurotrophic factor分类
医药卫生引用本文复制引用
孟盼,柳卓,朱青,赵洪庆,张秀丽,雷昌,王宇红,杨蕙..百事乐胶囊对抑郁模型大鼠海马隔颞轴BDNF、NT-3、NGF表达的影响[J].中国临床药理学与治疗学,2017,22(4):418-423,6.基金项目
国家自然科学基金青年项目(81403387) （81403387）
湖南省教育厅一般项目(14C0862,15C1038) （14C0862,15C1038）
湖南中医药大学校级青年基金(99820001-147) （99820001-147）
中医内科学省部共建教育部重点实验室开放基金(ZYNK201503) （ZYNK201503）
