中国临床医学2017，Vol.24Issue(2)：161-165,5.DOI:10.12025/j.issn.1008-6358.2017.20170114
缺氧通过刺激胸腺基质淋巴细胞生成素分泌促进宫颈癌细胞体外侵袭
Hypoxia promotes the invasion of cervical cancer cells in vitro by stimulating secretion of TSLP
摘要
Abstract
Objective:To explore the effects of hypoxia and thymic stromal lymphopoietin (TSLP) on the invasion of cervical cancer cells in vitro.Methods:Enzyme linked immunosorbent assay (ELISA) was performed to analyze the effects of normoxia and hypoxia on the secretion level of TSLP, and to analyze the effects of recombinant human TSLP (rhTSLP) protein on the expression levels of matrix metalloprotein-2 (MMP-2), MMP-9, and IL-8 in cervical cancer cell lines HeLa and CaSki.Matrigel invasion assay was used to investigate the effects of rhTSLP, hypoxia, and anti-human TSLP (aTSLP) neutralizing antibody (aTSLP, 5 μg/mL) on the invasiveness of HeLa and CaSki cells in vitro.Results:Under hypoxia exposure, the levels of TSLP secretion in HeLa and CaSki cells were significantly increased (P<0.01).RhTSLP (100 ng/mL) stimulation in vitro significantly enhanced the invasiveness of HeLa and CaSki cells (P<0.001), and promoted the productions of MMP-2, MMP-9, and IL-8 (P<0.01).The invasiveness of HeLa and CaSki cells can be promoted significantly under hypoxia exposure (P<0.001).In contrast, aTSLP (5 μg/mL) inhibited the invasiveness of HeLa and CaSki cells (P<0.05), and partly inhibited the stimulatory effect of hypoxia on invasiveness of HeLa and CaSki cells (P<0.05).Conclusions:Hypoxia facilitates the invasion of cervical cancer cells in vitro by up-regulating the secretion of TSLP.关键词
胸腺基质淋巴细胞生成素/缺氧/宫颈癌/侵袭Key words
thymic stromal lymphopoietin/hypoxia/cervical cancer/invasion分类
医药卫生引用本文复制引用
常凯凯,杨慧丽,周文洁,李明清,谢锋..缺氧通过刺激胸腺基质淋巴细胞生成素分泌促进宫颈癌细胞体外侵袭[J].中国临床医学,2017,24(2):161-165,5.基金项目
国家自然科学基金(81302260),上海市自然科学基金(17ZR1403200).Supported by National Natural Science Foundation of China (81302260) and Natural Science Foundation of Shanghai (17ZR1403200). （81302260）
