中国临床医学2017，Vol.24Issue(2)：166-170,5.DOI:10.12025/j.issn.1008-6358.2017.20160674
丹参酮ⅡA磺酸钠在缺血性斑马鱼模型中的促血管新生作用
The angiogenic effect and potential mechanism of sodium tanshinone ⅡA sulfonate in ischemic zebrafish model
陈飞 1乔祺 1王张生 1许澎 1陆刚2
作者信息
- 1. 复旦大学附属上海市第五人民医院心血管内科,上海 200240
- 2. 复旦大学附属上海市第五人民医院急诊科,上海 200240
- 折叠
摘要
Abstract
Objective:To investigate the angiogenic effect and potential mechanism of sodium tanshinone ⅡA sulfonate (Tan ⅡA) in ischemic zebrafish model.Methods:Vascular endothelial growth factor receptor kinase inhibitor (VRKI) was used to induce vascular injury in zebrafish model.Then the ischemic zebrafish model was treated with 50, 100, 200 μmol/L Tan ⅡA, respectively.The effects of Tan ⅡA on ischemic zebrafish subintestinal veins (SIVs) and intersegmental vessels (ISVs) were observed.Meanwhile, the expression changes of three vascular endothelial growth factor (VEGF) signaling pathway-related genes (flt-1, flk-1A, flk-1B) were detected by real-time PCR.Results:In VRKI-induced vascular injury zebrafish model, Tan ⅡA gradually restored ISVs and SIVs in a concentration-dependent manner, reversed the down-regulated expressions of three key VEGF signaling pathway-related genes (flt-1, flk-1A, flk-1B), and promoted angiogenesis.Conclusions:In the ischemic zebrafish model, Tan ⅡA plays a role in angiogenesis and vascular protection by regulating VEGF signaling pathway.关键词
丹参酮ⅡA磺酸钠/血管新生/斑马鱼/血管内皮生长因子Key words
sodium tanshinonⅡA sulfonate/angiogenesis/zebrafish/vasculaendothelial growth factor分类
医药卫生引用本文复制引用
陈飞,乔祺,王张生,许澎,陆刚..丹参酮ⅡA磺酸钠在缺血性斑马鱼模型中的促血管新生作用[J].中国临床医学,2017,24(2):166-170,5.
