中国人兽共患病学报2017，Vol.33Issue(4)：312-315,4.DOI:10.3969/j.issn.1002-2694.2017.04.004
新疆中哈边境地区分离到远东型和西伯利亚型蜱传脑炎病毒
Isolation of tick-borne encephalitis virus Far-eastern subtype and Siberian subtype in the China-Kazakhstan border area in Xinjiang
摘要
Abstract
To investigate the genotype and the biological characteristics of Tick-borne encephalitis virus (TBEV) in Charles Hilary endemic foci in the China-Kazakhstan border area in Xinjiang,ticks were collected by flagging during May to June in 2012 and 2014,and were stored in liquid nitrogen.TBEV strains were isolated from tick samples by inoculating BALB/c mice and BHK-21 cells.The FE gene fragments of TBEV-Far and the S gene fragments of TBEV-Sib were detected by RT-PCR from infected mice brain tissue and BHK cells,and then subjected to sequence alignment.Totally 16 TBEV strains were isolated from Ixodes persulcatus and Dermuceuter silvarum,among 13 strains were Far eastern subtype,three strains were Siberian subtype.It was first time that the TBEV-Sib was isolated in China.The Charles Hilary TBE natural foci were in the China-Kazakhstan border area,and both TBEV-Far and TBEV-Sib co-circulated.关键词
蜱传脑炎病毒/远东型/西伯利亚型/疫源地/中哈边境Key words
Tick-borne encephalitis virus/TBEV-Far/TBEV-Sib/endemic foci/the border areas near Kazakhstan分类
医药卫生引用本文复制引用
张桂林,孙响,刘然,郑重,刘晓明,伊小平..新疆中哈边境地区分离到远东型和西伯利亚型蜱传脑炎病毒[J].中国人兽共患病学报,2017,33(4):312-315,4.基金项目
国家自然科学基金项目(No.U1303104)Supported by the National Natural Science Foundation of China (No.U1303104) （No.U1303104）
