中国人兽共患病学报2017，Vol.33Issue(4)：316-320,5.DOI:10.3969/j.issn.1002-2694.2017.04.005
不同地理种群白纹伊蚊线粒体基因COI的遗传多样性分析
Genetic diversities of different geographical populations of Aedes albopictus based on mitochondrial gene COI
摘要
Abstract
In order to figure out phylogenetic relationship and genetic diversity of different geographical populations,genetic analyses of Aedes albopictus were performed based on mitochondrial gene COI.Based on samples collected from most distribution regions in China,mitochondrial gene Cytochrome C Oxidase Subunit I was obtained through PCR and DNA sequence.Together with some COI sequences downloaded from GenBank,60 COI sequences with the final length of 598 bp were used for subsequent analyses.Results showed that there was no obvious divergence according to phylogenetic analyse,all sequences were clustered together in Maximum Likelihood tree.Sixteen haplotypes were detected,and four of them shared haplotypes.Haplotype diversity (Hd) was 0.737,nucleotide diversity (π) was 0.20 ％.Population genetic differentiation analyses demon strated that Hainan population showed obvious divergences.In the network of haplotypes,H1 and H6 was found to be the primary haplotypes,and they formed two radical centers.All these results indicate that A.albopictus populations of China are expanding presently,and Hainan population become differential with other geographical populations,which probably attribute to geographical isolations.关键词
白纹伊蚊/地理隔离/瓶颈效应/蚊媒病Key words
Aedes albopictus/geographical isolation/bottleneck effect/mosquito-borne disease分类
医药卫生引用本文复制引用
张瑞玲,姚广琴,潘晓倩,马德珍,赵爱华,张忠..不同地理种群白纹伊蚊线粒体基因COI的遗传多样性分析[J].中国人兽共患病学报,2017,33(4):316-320,5.基金项目
国家自然科学基金(Nos.81401693&81572028)和大学生创新创业训练计划(No.201510439010)联合资助Supported by the Natural Science Foundation of China (Nos.81401693 & 81572028) and the Student's Platform for Innovation and Entrepreneurship Training Program (No.201510439010) （Nos.81401693&81572028）
