中国人兽共患病学报2017，Vol.33Issue(4)：321-325,5.DOI:10.3969/j.issn.1002-2694.2017.04.006
昆明市腹泻人群肠致病性大肠杆菌流行特征研究
Epidemiological characteristics investigation of enteropathogenic Escherichia coli in diarrhea patients from Kunming City, China
摘要
Abstract
In order to provide scientific reference for the prevention and treatment of EPEC infection,the epidemiological characteristics of enteropathogenic Escherichia coli (EPEC) was investigated in Kunming City,Yunnan.A total of 1 121 diarrhea patients and 319 healthy controls were recruited from four sentinel hospitals in Kunming City from June 2014 to July 2015.The stool samples were cultured and the total DNA was extracted to detect EPEC with real-time polymerase chain reaction (PCR).A structured questionnaire was applied to each case for the clinical manifestation and the basic information.Results showed that the detection rate of EPEC in diarrhea patients was more than the controls (5.53％,1.88％,x2 =7.36,P＜0.05).The prevalence of EPEC was no significant difference between male and female in diarrhea patients (5.54％,5.53％,x2=0.001,P=0.999).However,the detection rate of EPEC had significant difference in different age groups (x2 =17.27,P＜0.05),among which the detection rate was the highest in 2-5 years group (12.12％,16/ 132).And the prevalence of EPEC showed remarkable seasonal trend in diarrhea cases (x2 =12.82,P＜0.05),and the detection rate was the highest in summer (9.00％,18/200) and the lowest in winner (3.05％,12/394).Watery stool was found in 61.29％ (38/62) in diarrhea cases with EPEC infection,and dehydration was found in 12.9 ％ (8/62) in diarrhea cases associated with EPEC infection.Results showed EPEC is one of the mainly pathogens causing diarrhea in Kunming City,and EPEC infection occurred mainly in children under 5 years,and it is prone to dehydration.关键词
腹泻病例/非腹泻研究对象/肠致病性大肠杆菌/流行病学特征Key words
diarrhea patient/healthy control cases/EPEC/epidemiological characteristics分类
医药卫生引用本文复制引用
杨春利,陈军虎,李石柱,陈家旭,周晓农,张顺先,艾琳,蔡玉春,卢艳,陈韶红,徐闻,古文鹏,胡薇..昆明市腹泻人群肠致病性大肠杆菌流行特征研究[J].中国人兽共患病学报,2017,33(4):321-325,5.基金项目
国家科技重大专项(No.2008ZX10004-011 （）
No.2012ZX10004-220)、国家卫生行业科研专项(No.201502021)和上海市公共卫生第四轮三年行动计划(GWIV-29)联合资助 Supported by the National Science and Technology Major Project (Nos.2008ZX10004-011 and 2012ZX10004-220),the Chinese Special Program for Scientific Research of Public Health (No.201502021),and the Fourth Round Three Year Action Plan Public Health of Shanghai,China (No.GWIV-29) （No.201502021）
