中国人兽共患病学报2017，Vol.33Issue(4)：326-331,342,7.DOI:10.3969/j.issn.1002-2694.2017.04.007
半胱氨酸天冬氨酸蛋白酶3在亚洲带绦虫实验感染乳猪肝组织的表达
Expression of cysteine aspartyl proteinase 3 from liver tissues of the porkets experimentally infected by Taenia asiatica
摘要
Abstract
In order to provide substantial scientific information for exploring the mechanism of porcine liver injury caused by Taenia asiatica (T.asiatica),the expression of Cysteine aspartyl proteinase 3 (Caspase-3) from liver tissues of porkets that were experimentally infected by T.asiatica was examined.The T.asiatica adults were collected from the taeniasis patients in Duyun,Guizhou Province and identified biologically.The eggs were harvested from gravid proglottids and prepared by repeated washing and centrifugation.Twelve 20-days old Yorkshire and Seghers hybrid porkets were randomly divided into experimental and control groups as six pigs per group.The experimental group was orally administrated with 1.5 × 106 eggs per porket at day 0 post-infection.The porkets of both groups were sacrificed on the day 15 and day 75 post-infection (three pigs per time point) respectively,and liver samples were collected for further experiments.Quantitative real-time polymerase chain reaction method was employed to detect the mRNA levels of Caspase-3,and western blotting and immunohistochemistry methods were performed to detect the level of Caspase-3 expression in both groups.At the day 15 post-infection,the mRNA level and expression level of Caspase-3 of the experimental group were significantly decreased,comparison with the control group (P =0.011,P=0.008 and P=0.004 respectively).It was positive with Caspase-3 when yellow or brown signal appeared in the cytoplasm of liver cells by immunohistochemistry.However,at the day 75 post-infection,the mRNA level and expression level of Caspase-3 of the experimental group were dramatically similar to the control group.Furthermore,in the experimental group,the mRNA level and expression level of Caspase-3 were significantly increased at day 75 post-infection than day 15 post-infection (P--0.018,P=0.003 and P=0.002 respectively).These results suggested that Caspase-3 might be involved into the regulation of the damage of porcine liver induced by T.asiatica challenge at the early infection stage and have on effect to the hepatic injury because of the dramatic recovery of Caspase-3 at the consequent infection stage.关键词
亚洲带绦虫/半胱氨酸天冬氨酸蛋白酶3/乳猪/肝脏Key words
Taenia asiatica/cysteine aspartyl proteinase 3/porket/liver分类
医药卫生引用本文复制引用
许士刚,牟荣,张科,杨林,郎书源,包怀恩..半胱氨酸天冬氨酸蛋白酶3在亚洲带绦虫实验感染乳猪肝组织的表达[J].中国人兽共患病学报,2017,33(4):326-331,342,7.基金项目
国家自然科学基金项目(No.81160205)、贵州省优秀科技教育人才省长资金项目(黔省专合字[2008]55号)和贵州省留学人员科技创新项目(黔人项目资助合同[2015]1号)联合资助 Supported by the National Natural Science Foundation of China (No.81160205),the Specific Fund of Provincial Government in Guizhou Province (No.[2008]55),and the Science and Innovation Foundation of Guizhou Province for Returned Chinese Scholars (No.[2015]1) （No.81160205）
