中国人兽共患病学报2017，Vol.33Issue(4)：332-336,5.DOI:10.3969/j.issn.1002-2694.2017.04.008
青海省251株结核分枝杆菌Spoligotyping基因型与4种一线药物耐药表型的研究
Investigation on spoligotyping and phenotypes of drug resistance to four first-line drugs in 251 Mycobacterium tuberculosis isolates from Qinghai, China
摘要
Abstract
This study aims to learn drug resistance situation of four first-line anti TB drugs among 251 Mycobacterium tuberculosis isolates from Qinghai and to explore their relationships with genotypes by Spoligotyping,so as to provide basis for effective prevention of tuberculosis.Isolates of 251 M.tuberculosis were tested susceptibilities of four first-line drugs including isoniazid (INH),rifampicin (RFP),streptomycin(SM) and ethambutol (EMB) by using conventional proportion method and genotyped by Spoligotyping.Relationship between drug resistance and genotypes was analyzed statistically.Results showed the total drug resistance rate was 56.2％ (141/251) among 251 M.tuberculosis isolates.Resistance rates of four first-line drugs were 43.0％ (108/251) for INH,37.1％ (93/251) for RFP,39.0％ (98/251) for SM,27.9％ (70/251) for EMB respectively.Rate of multidrug-resistant TB (MDR TB) was 31.5％ (79/251).All 251 isolates of M.tuberculosis were typed by spoligotyping.The 185 (73.7 ％) were Beijing genotypes and 66 (26.3.％) were non-Beijing genotypes,and no statistical association was found with drug resistance.This paper concludes that isolates of M.tuberculosis prevail in Qinghai have both high rates of drug resistance and MDR and dominant isolates are Beijing genotypes by spoligotyping.关键词
结核分枝杆菌/耐药/寡核苷酸序列分型Key words
Mycobacterium tuberculosis/drug resistance/spoligotyping分类
医药卫生引用本文复制引用
王兆芬,王新华,李斌,蒋明霞,马永成,汪海静,申秀丽,姚雪琼,李婷婷,陈英..青海省251株结核分枝杆菌Spoligotyping基因型与4种一线药物耐药表型的研究[J].中国人兽共患病学报,2017,33(4):332-336,5.基金项目
国家自然科学基金(No.81660556) （No.81660556）
青海省科技厅项目(No.2014-ZJ-910)联合资助 Supported by the National Natural Science Foundation (No.81660556) and Qinghai Science and Technology Commission (No.2014-ZJ-910) （No.2014-ZJ-910）
