中国人兽共患病学报2017，Vol.33Issue(4)：343-348,6.DOI:10.3969/j.issn.1002-2694.2017.04.010
首次自褐家鼠粪便标本中检出香港海鸥菌
First isolation of Laribacter hongkongensis from stool samples of Rattus norvegicus
摘要
Abstract
In order to investigate whether Laribacter hongkongensis could be detected in stool samples of Rattus norvegicus in the wild,Rattus norvegicus were trapped alive in an urban community of Guangzhou,China over a period of one year from June 2015 to May 2016,and their stool samples were examined for the presence of L.hongkongensis strains.Isolates were identified based on phenotypic characteristics and 16S rRNA sequence analysis,and were examined for their susceptibility to 19 antimicrobial agents.Further typing of the isolates was performed using multi-loci sequence typing (MLST) analysis.A total of 191 R.norvegicus were trapped alive.L.hongkongensis was identified and successfully isolated from two samples,representing a prevalence of 1.05 ％.Although the two isolates possessed similar phenotypic characteristics and have no base difference of 16S rRNA gene,they constituted two new distinct sequence types (STs),ST-163 and ST-164.This is the first report that L.hongkongensis can be detected in the intestinal tract of R.norvegicus.Results suggest that R.norvegicus could serve as carriers of L.hongkongensis and therefore could be another potential source of infection.关键词
香港海鸥菌/褐家鼠/16S rRNA测序/多位点序列分型Key words
Laribacter hongkongensis/Rattus norvegicus/16S rRNA sequence/sequence type分类
医药卫生引用本文复制引用
刘优招,陈炜聪,刘志华,王丽,葛婧,韩宇宇,陈清,胡静..首次自褐家鼠粪便标本中检出香港海鸥菌[J].中国人兽共患病学报,2017,33(4):343-348,6.基金项目
国家自然科学基金(No.81373052&81373051)资助 Supported by the National Science Foundation of China (Nos.81373052 & 81373051) （No.81373052&81373051）
