中国水产科学2017，Vol.24Issue(3)：432-439,8.DOI:10.3724/SP.J.1118.2017.16325
基于线粒体Cytb基因的虾夷扇贝群体遗传结构分析
Population-genetics analysis of the Japanese scallop Patinopecten yessoensis based on mitochondrial Cytb gene
摘要
Abstract
The introduced Japanese scallop Patinopecten yessoensis has been farmed in China, primarily along the coasts of the Bohai Sea and the North Yellow Sea, since the 1980s. Following the adaptation and localization ofP. yessoensis in China, specific problems have appeared after several decades of aquafarming: especially low sur-vival and slower grow. This can be associated with an effectively small population that experiences inbreeding, eventually leading to unknown degrees of decline in its genetic diversity after several generations. Consequently, it is important to carry out genetic diversity research onP. yessoensis in its main mariculture area of China. In this study, mitochondrial cytochrome-b (Cytb) gene-sequencing techniques were used to determine genetic diversity and variations among six stocks ofP. yessoensis:the bottom-sowing cultured stocks of Changdao, Haiyangdao and Zhangzidao; the natural stocks of Lüshun and Japan; and the artificial selected stock of Zhangzihong. A total of 20 haplotypes were found among 120 sequences of Cytb gene from the six stocks ofP. yessoensis. The minimum number of haplotypes was found in the Zhangzihong artificial breeding stock, and the maximum number occurred in the Japan Aomori Mutsu Bay stock, with a haplotype diversity (Hd) of 0.10000 and 0.88400, respectively. Analysis of molecular variance (AMOVA) showed the percentage of variation between the Japan and China groups was 15.34%, which was significantly higher than when measured as one genetic pool. For the China group, 83.41% of the genetic variation come from within the stocks, and only 1.52% of the variation was among the stocks in the group, indicating that genetic variation between individuals of the China group was higher than be-tween the stocks.Fst analysis showed a moderate level of genetic variance among the Japan stock and China stocks (0.07455?0.17895,Fst>0.05). The genetic distance between the six stocks ofP. yessoensis was calculated based on the Tamura-Nei model; the maximum genetic distance was found between the Japan Aomori Mutsu Bay stock and the Haiyangdao bottom-sowing cultured stock; the minimum genetic distance was found between the Zhangzidao bottom-sowing cultured stock and the Zhangzihong artificial breeding stock. The UPGMA method was used to construct a phylogenetic tree based on genetic distances among the six stocks ofP. yessoensis; the tree shows that the five China stocks clustered into a genetic branch, and then these clustered together with the Japan group. These results demonstrate obvious genetic differentiation between the China stocks and the Japan stock of this scallop. Moreover, genetic diversity was lower in theP. yessoensis China stocks as compared with the Japanese stock. The findings of this study could be usefully applied to scientific strategies for the protection, sustainable development and commercial utilization of scallop germplasm resources.关键词
虾夷扇贝/Cytb/遗传结构/遗传多样性/种质资源Key words
Patinopecten yessoensis/Cytbgene/genetic structure/genetic diversity/germplasm resources分类
农业科技引用本文复制引用
倪守胜,杨钰,柳淑芳,庄志猛..基于线粒体Cytb基因的虾夷扇贝群体遗传结构分析[J].中国水产科学,2017,24(3):432-439,8.基金项目
"十二五"农村领域国家科技计划项目(2012BAD18B03) （2012BAD18B03）
山东省泰山学者建设工程专项. （）
