中国输血杂志2017，Vol.30Issue(3)：247-250,4.DOI:10.13303/j.cjbt.issn.1004-549x.2017.03.008
输注储存血液对黑色素瘤荷瘤小鼠辅助性T细胞1和2平衡的影响
Effects of storage blood transfusion on balance of helper T cell 1 and 2 in melanoma bearing mice
摘要
Abstract
Objective To investigate the effects of blood transfusion on TH1/TH2 balance and expressions of cytokines in melanoma-bearing mice.Methods The tumor model of melanoma mice was established.The fresh blood group and stored blood group were set up (30 mice/group).Mice were transferred with 0.2 mL fresh blood (D0) or stored blood(D15) via tail vein.The whole blood in mice were collected at 15 minutes,30 minutes,1 hour,4 hours and 8 hours after blood transfusion respectively.The expression levels of cytokines (TNF-α,INF-γ,IL-2,IL-4,IL-10) in plasma at each time point were detected by cytometric beads array (CBA).Control group was also set up without blood transfusion (6 mice/group).Results 1) The secretion levels of TNF-α(pg/mL) were 9.14±0.83 in the fresh group and 1.72±0.90 in the control group respectively at 4 hour after blood transfusion (P＜0.01).The expression levels of IL-4(pg/mL) were1.55±0.17 in the stored group and 0.29±0.08 in the control group respectively at 4 hour after blood transfusion (P＜0.01).The expression levels of IL-10 (pg/mL) were 5.4±1.39 in the stored group and 0.2±0.08 in the control group respectively at 4 hour after blood transfusion (P＜0.01).2) The expression levels of TNF-α(pg/mL) were 3.67± 1.76 in the stored group and 7.64± 1.04 in the fresh group respectively at 1 hour after blood transfusion (P＜0.05).The expression levels of INF-γ(pg/mL) were 0.27±0.16 in the stored group and 0.49±0.18 in the fresh group respectively at 1 hour after blood transfusion (P＜0.05).The expression levels of IL-2 (pg/mL) were 0.29±0.08 in the stored group and 0.89±0.21 in the fresh group respectively at 4 hours after blood transfusion (P＜0.01).The expression levels of IL-10 (pg/mL) were 5.40± 1.39 in the stored group and 2.38±0.54 in the fresh group respectively at 4 hours after blood transfusion (P＜0.01).Conclusion Blood transfusion promotes the expressions of TH1 and TH2 cytokines.Compared with the infusion of fresh blood,infusing stored blood promotes the expressions of TH2 cytokines and the balance of TH1/TH2 in infusing stored blood group shifts to TH2 dominance.关键词
异体输血/储存血/新鲜血/黑色素瘤/TH1/TH2平衡/输血相关免疫调节/小鼠/动物实验Key words
allogeneic blood transfusion/storage blood/fresh blood/melanoma/TH1/TH2 balance/transfusion related immunomodulation/mice/animal experiment分类
医药卫生引用本文复制引用
张敬军,严育忠,朱鑫方,陈淑英,张琦,夏荣..输注储存血液对黑色素瘤荷瘤小鼠辅助性T细胞1和2平衡的影响[J].中国输血杂志,2017,30(3):247-250,4.基金项目
国家自然科学基金面上项目(81570166,81170530) （81570166,81170530）
