中国输血杂志2017，Vol.30Issue(3)：251-254,4.DOI:10.13303/j.cjbt.issn.1004-549x.2017.03.009
红细胞储存损伤对于大肠杆菌感染大鼠输注效果的影响
The influence of erythrocyte storage lesion on transfusion effects for rats infected by Escherichia Coli
摘要
Abstract
Objective To investigate the influence of storage lesion of red blood cells (RBCs) on mortality of SpragueDawley rats infected by Escherichia Coli (E.coli).Methods 69 SPF-SD rats,according to use of cyclophosphamide and dexamethasone or transfusion with RBCs stored 9 days or less than 1 day or with normal saline (NS),were randomly divided into four groups:normal + NS;immunosuppression + 9d RBCs;immunosuppression + ld RBCs:immunosuppression +NS,and control group.Each of three immunosuppressive groups contained 18 rats,while the control group had 15 only.The rats,subjected to bloodletting 20％ of total blood volume,were transfused with RBCs stored nine days,or less than one day or with NS,separately,and then were injected with E.coli (0.5 mL) through femoral vein.When transfused 24 h later,the blood of rats were collected from tail vein to determine serum iron and blood bacteria culture,and three rats of every immunosuppressive group were killed to make pathological specimen.Results For the immunosuppressive groups transfused 9d RBCs,ld RBCs,NS and control group,the mortality were 10/15,11/15 and 9/15,respectively (P＞0.05).The mean survival time (h) were 55.6±21.3,38± 16.6 and 28.3± 15.0,respectively (P＜0.05).The serum iron ion concentration (μmol/L)were 34.1±9.8,28± 10.2,26.8±9.0 and 22.4±5.9,respectively (P＞0.05).Meanwhile,the values (OD =600 nm) of blood culture were 0.510±0.340,0.790±0.281,0.427±0.107 and 0.349±0.069,respectively (P＜0.05).Conclusion No statistical difference in mortality of immunosuppressive rats infected by E.coli were found,when they were transfused with RBCs of stored different periods,separately.Nevertheless,infusion of RBCs with shorter storage period,compared with RBCs stored for longer time,promotes the proliferation of E.coli in immunosuppressive rats,and accelerates the death process of rats.关键词
输血/红细胞/储存损伤/大肠杆菌/免疫抑制/SD大鼠/动物实验Key words
transfusion/red blood cell/storage lesion/Escherichia Coli/immunosuppression/S-D rats/animal research分类
医药卫生引用本文复制引用
王向明,于浩,刘忠,焦伯海,王珏,尹湧华,宋宁,田力,李玲,孔玉洁,欧国进..红细胞储存损伤对于大肠杆菌感染大鼠输注效果的影响[J].中国输血杂志,2017,30(3):251-254,4.基金项目
国家自然科学基金面上项目(81370669),中国医学科学院医学与健康科技创新工程(2016-I2M-3-024),北京协和医学院教改项目(10023201601901)和四川省科技厅科技计划项目(2015JY0121) （81370669）
