中国输血杂志2017，Vol.30Issue(3)：255-258,4.DOI:10.13303/j.cjbt.issn.1004-549x.2017.03.010
干扰素刺激基因15抑制严重发热伴血小板减少综合征病毒复制的初步研究
Study on the role of interferon-stimulated gene 15 (ISG15) to inhibit the replication of severe fever with thrombocytopenia syndrome virus
摘要
Abstract
Objective To analyze the impact of interferon-stimulated gene 15 (ISG15) on the replication of SFTSV.Methods To construct a plasmid with over-expression of ISG15,ISG15 gene was amplified from Hela cell using reverse transcription (RT)-PCR and then cloned in the plasmid.The effect of ISG15 expression on SFTSV replication was evaluated by transfecting over-expressed ISG15 plasmid on Hela cells.The effect of ISG15 over-expression on SFTSV replication was evaluated after infection with SFTSV using quantitative RT-PCR.Results ISG15 was expressed successfully in Hela cells.Hela cell supported the replication of SFTSV.The over-expression of ISG15 might inhibit the replication of SFTSV.Conclusion The over-expressed ISG15 can inhibit the replication of SFTSV.However,its mechanism needs to be further explored.关键词
干扰性刺激基因15/血小板减少综合征/严重发热/病毒复制/基因克隆/PCR,实时荧光定量Key words
ISG15/SFTS/severe fever/virus replication/gene clone/PCR/real-time fluorescence quantification分类
生物学引用本文复制引用
陈旭,李世林,曾沛斌,陈利民..干扰素刺激基因15抑制严重发热伴血小板减少综合征病毒复制的初步研究[J].中国输血杂志,2017,30(3):255-258,4.基金项目
国家自然科学基金(81400096),成都市科技局科技惠民技术研发项目(2015-HM01-00214-SF)和中国医学科学院医学与健康创新工程项目(2016-I2M-3-025) （81400096）
