中国兽医科学2017，Vol.47Issue(5)：551-556,6.DOI:10.16656/j.issn.1673-4696.2017.05.003
牛伯氏疏螺旋体感染的间接ELISA检测方法的建立
Development of an indirect ELISA for the detection of Borrelia burgdorferi infecion in cattle
摘要
Abstract
An indirect ELISA for diagnozing Borrelia burgdorferi-infected cattle was established by using soluble whole bacterial antigen prepared from Borrelia burgdorferi sensustricto strain B31,and its specificity and sensitivity were evaluated.The ELISA developed in this study could identify a Lyme disease-positive bovine serum that was 800-fold diluted.A total of 200 clinical samples and 300 known samples were evaluated for the ELISA method and immunofluorescence assay(IFA),and the coincidence rate between the two methods was 100％ in detecting 200 clinical samples,and 81.8％ in detecting 300 known samples,respectively.These results indicated that our ELISA would be useful for diagnosis of bovine infected with Borrelia burgdorferi.关键词
莱姆病/伯氏疏螺旋体/间接酶联免疫吸附试验Key words
Lyme disease/Borrelia burgdorferi/indirect ELISA分类
农业科技引用本文复制引用
梅琳,王慧煜,李佳,吴绍强,林祥梅,关贵全,郝琴,韩雪清..牛伯氏疏螺旋体感染的间接ELISA检测方法的建立[J].中国兽医科学,2017,47(5):551-556,6.基金项目
虫媒性外来动物疫病传播阻断技术研究项目(2013BAD12B03) （2013BAD12B03）
