中国兽医科学2017，Vol.47Issue(5)：582-586,5.DOI:10.16656/j.issn.1673-4696.2017.05.008
猪带绦虫Ddi1-like蛋白的真核表达及其抗血清的制备
Eukaryotic expression and anti-serum preparation of Ddi1-like protein from Taenia solium
摘要
Abstract
To functionally study the DNA damage-inducible-like (Ddi1-like) protein from Teania solium,according to gene annotations of T.solum genome in GeneDB,the cDNA of Ddi1-like gene was amplified by RACE (rapid-amplification of cDNAends) technique using the specific 5'and 3'primers,and was then cloned into vecter pPIC9K.Then the recombinant protein was induced in P.pastoris.New Zealand white rabbits were immuned by the purified recombinant Ddi1-like protein to produce the anti-serum.In result,the full ORF of Ddi1-like gene was successfully obtained.The recombinant Ddi1-like protein was produced in P.pastoris expression system.The recombinant protein expression was confirmed by SDS-PAGE and Western-blot analysis.The antibody titer of the anti-serum could reach to 1∶12 800.The above-mentioned results lay a foundation to further studies on the proteolytic activity of the Ddi1-like protein.关键词
绦虫/Ddi1-like蛋白/真核表达/蛋白酶Key words
tapeworms/Ddi1-like protein/eukaryotic expression/proteinases分类
农业科技引用本文复制引用
何伟,殷静,岳生赟,刘仲藜,王帅,才学鹏..猪带绦虫Ddi1-like蛋白的真核表达及其抗血清的制备[J].中国兽医科学,2017,47(5):582-586,5.基金项目
国家重点基础研究发展计划(973)项目(2015CB150300) （973）
