中国兽医科学2017，Vol.47Issue(5)：592-596,5.DOI:10.16656/j.issn.1673-4696.2017.05.010
狂犬病病毒G蛋白V区特异性单克隆抗体的制备与鉴定
Preparation and identification of monoclonal antibody specific to the V region of rabies virus G protein
摘要
Abstract
In order to prepare monoclonal ant ibody(McAb) against the glycoprotein(G protein) of rabies virus(RV) and identify its binding ability in this study,we used the polypeptide"PPDQLVNLHDFRSDEIEHLVVEE"conjugated to KLH to immune mice.A hybridoma strain designated 4D3 was successfully prepared,which belonged to IgG2b subclass with a light κ chain.The eukaryotic expression vector pCAGGS-G/pCAG-GS-13G were constructed and G protein was transiently expressed in vitro.The McAb secreted by 4D3,as analyzed by Western blot and immunofluorescence assay,specifically reacted with the eukaryotically expressed G protein of the attenuated RV strain LBNSE,but not with that of virulent strain IMDRV-13.The McAb prepared in this study thus constitute a useful tool for identification of field isolates of rabies virus and for structural and functional investigation of the G protein.关键词
狂犬病病毒/糖蛋白/真核表达/单克隆抗体Key words
rabies/glycoprotein/eukaryotic expression/monoclonal antibody分类
农业科技引用本文复制引用
汪孟航,温永俊,朱洪伟,刘莹,刘杏,李新培,王西西,杨勇,孙娜,程世鹏..狂犬病病毒G蛋白V区特异性单克隆抗体的制备与鉴定[J].中国兽医科学,2017,47(5):592-596,5.基金项目
公益性行业(农业)科研专项(201303042) （农业）
国家自然科学基金面上项目(31572505) （31572505）
