中国兽医科学2017，Vol.47Issue(5)：597-602,6.DOI:10.16656/j.issn.1673-4696.2017.05.011
水貂肠炎病毒VP2基因的原核表达及多克隆抗体的制备
Prokaryotic expression of VP2 gene of mink enteritis virus and preparation of anti-serum
摘要
Abstract
The study aims to express the capsid protein VP2 of mink parvovirus in Escherichia coli(E.coli) and prepare its anti-serum.At first,according to the sequence of VP2 gene,two specific primers were designed.The recombinant plasmid pB-MEV-L with full length MEV genome was used as template in PCR amplification.After insertion of VP2 gene into prokaryotic expression vector pET-32a,the recombinant plasmid pET-MEV-VP2 was constructed successfully.Then transformation of pET-MEV-VP2 into E.coli BL21(DE3) and high expression was induced by IPTG.After 4 times immunization with the purified VP2 protein,the antiserum titer for ELISA was 1:1 024.The result of immunofluorescence assay showed that the rabbit antiserum had a good combined activity.All the results laid a solid foundation for further study of the biological characteristics of MEV.关键词
水貂肠炎病毒/VP2/原核表达/抗血清Key words
mink enteritis virus/VP2/prokaryotic expression/antiserum分类
农业科技引用本文复制引用
卞赛赛,梁琳,高窦,周灵,李沛然,杨双双,雷程红,崔尚金,刘维全..水貂肠炎病毒VP2基因的原核表达及多克隆抗体的制备[J].中国兽医科学,2017,47(5):597-602,6.基金项目
国家高技术研究发展计划(863)项目(2011AA10A213) （863）
中国农业科学院创新工程项目(ASTIP-IAS15) （ASTIP-IAS15）
