中国兽医科学2017，Vol.47Issue(5)：644-648,5.DOI:10.16656/j.issn.1673-4696.2017.05.019
奥尔森派琴虫PCR检测方法的优化及其在进出境检疫中的应用
Optimization of PCR detection for Perkinsus olseni and its application in entry and exit quarantine
摘要
Abstract
To establish a rapid and specific PCR method to detect Perkinsus olseni and prevent this harmful parasite from spreading into the country,the PCR detection method recommended by the World Organisation forAnimal Health(OIE) was optimized and then applied for detection of 95 batches of samples collected from 21 types of shellfish products from 16 countries.The result showed that without the change of sensibility,the optimized PCR procedure has decreased the time from 224 min to 91 min.The efficiency of the optimized PCR procedure has increased to 2.6 times.P.olseni was detected in 77.8％(7/9) scallops imported from Australia.The sequence analysis revealed that the homology was 99％(U07701.1).The results showed that the optimized PCR method met requirements for use in rapid testing of P.olseni.Moreover the aquatic products imported from Australia should be detected carefully.关键词
奥尔森派琴虫/PCR检测/水产品/澳大利亚Key words
Perkinsus olseni/PCR detection/aquatic product/Australia分类
农业科技引用本文复制引用
李树清,何宇平,王艳,陈志飞,王巧全,李春阳,张强,林颖峥,吴成云,唐智芳..奥尔森派琴虫PCR检测方法的优化及其在进出境检疫中的应用[J].中国兽医科学,2017,47(5):644-648,5.基金项目
国家重点研发计划项目(2016YFC1202000,2016YFC1202004) （2016YFC1202000,2016YFC1202004）
