中国现代医学杂志2017，Vol.27Issue(8)：1-6,6.DOI:10.3969/j.issn.1005-8982.2017.08.001
不同剂量链脲佐菌素诱导C57BL/6小鼠1型糖尿病模型的复制与iNKT细胞的检测
Study on model of type 1 diabetes in C57BL/6 mice induced by different dose of streptozotocin and detection of iNKT cell
摘要
Abstract
Objective To establish an animal model of type 1 diabetes mellitus similar to human and study the modeling optimal dose of streptozotocin (STZ). Methods A total of 35 female C57BL/6 mice were randomly divided into control group ( =5) and model group1, 2, 3 (the dose of STZ was 40 mg/kg, 50 mg/kg, 60 mg/kg respectively) with 10 mice in each group. By intraperitoneal injection to the model group of different doses of STZ for 5 days. Blood glucose and body weight before and 1, 2, 3, 4, 5 weeks after injection were determined. Possitive rate of insulin autoantibodies (IAA) in mice were determined. The conditions of mice eating, drinking and urination were observed. Flow cytometry (FACS) analysis was used to analyze the proportion of iNKT cells in blood and spleen cell suspension. HE staining was used to observe the pathological changes of pancreatic islets. Results Compared with the control group, the amount of drinking water, food intake and urine volume were significantly increased, and the weight was significantly reduced in model group 3. Compared with the control group, the change of blood glucose in model group 3 was obvious, and the first week after injection of STZ the blood glucose increased rapidly, and reached its peak in the fourth week and then de-creased, the difference was significant ( <0.05). Compared with the control group, the three groups of model group were significantly decreased in the number of islet cells,. The positive rates of IAA in model group 2 and 3 were 30% and 90% respectively. Compared with the control group, the proportion of CD4+ NK1.1+ lym-phocytes in peripheral blood and CD4+ NK1.1+ lymphocytes in spleen were significantly decreased in model group 3, and the differences were significant ( < 0.05). Conclusions The optimal dose of STZ is 60 mg/kg in inducing and establishing of type 1 diabetes mellitus in female C57BL/6 mice.关键词
1型糖尿病/链脲佐菌素/C57BL/6小鼠/恒定自然杀伤T细胞Key words
Type 1 diabetes mellitus/streptozotocin/C57BL/6 mice/iNKT cell分类
医药卫生引用本文复制引用
陈冬志,尹晓琳,娄永富,杨飞,王园园,刘嘉琳,孟明,侯明辉..不同剂量链脲佐菌素诱导C57BL/6小鼠1型糖尿病模型的复制与iNKT细胞的检测[J].中国现代医学杂志,2017,27(8):1-6,6.基金项目
国家自然科学基金(No:81373197) （No:81373197）
河北省自然科学基金(No:H2014201133,No:H2015201131) （No:H2014201133,No:H2015201131）
河北大学医学学科建设项目(No:2014A1001) （No:2014A1001）
国家级大学生创新项目基金(No:201510075030) （No:201510075030）
