中国现代医学杂志2017，Vol.27Issue(8)：7-11,5.DOI:10.3969/j.issn.1005-8982.2017.08.002
抑制核转录因子κBp65对鼻咽癌CNE2细胞的生物学影响
Inhibition of NF-κBp65 expression reduce malignant phenotype of nasopharyngeal carcinoma CNE2 cell
摘要
Abstract
Objective To investigate the biological effects of NF-kappa B p65 (NF-κBp65) on nasopha-ryngeal carcinoma. Methods NF-κBp65 specific siRNA plasmid expression vector (pGPU6/RFP/Neo-NF-κB p65) and negative control plasmid expression vector (pGPU6/RFP/Neo-NC) were constructed and transfected into human nasopharyngeal carcinoma CNE2 cell line by non-liposomal lipid transfection agent to establish NF-κB p65 silencing stable transfected CNE2 cell line (NF-κB p65 silencing group) and negative control cell line (negative control group). The expression of NF-κB p65 gene in cancer cells was analyzed by Western blot. The proliferation and cell cycle of cancer cells were analyzed by MTT assay and Flow Cytometry. A xenograft model in nude mice was established to analyze the tumor igenicity of cancer cells. Results The stable transfected CNE2 cell lines were successfully obtained. The expression of NF- κB p65 protein was de creased in cells of NF-κB p65 silencing group. The cell cycle was arrested in S phase and proliferation was reduced after NF-κB p65 was silenced. The growth of transplanted tumor in nude mice of NF-κB p65 silencing group was slower, and weight of transplanted tumor was lighter than that in negative control group or blank control group, the difference was statistically significant ( =14.386, <0.05). Conclusions Malignant biological phenotype of nasopharyngeal carcinoma CNE2 cell line could be reduced by silencing NF- κB p65.关键词
鼻咽癌/核转录因子-κBp65/小干扰核糖核酸Key words
nasopharyngeal carcinoma/NF-κB p65/siRNA分类
医药卫生引用本文复制引用
范才文,唐娟,黄辉,李璐,罗琴,易世江,向秋..抑制核转录因子κBp65对鼻咽癌CNE2细胞的生物学影响[J].中国现代医学杂志,2017,27(8):7-11,5.基金项目
国家自然科学基金资助课题(No:81260344) （No:81260344）
广西自然科学基金资助课题(No:2013GXNSFAA019228) （No:2013GXNSFAA019228）
广西壮族自治区教育厅资助课题(No:LX2014274) （No:LX2014274）
