中国现代医学杂志2017，Vol.27Issue(8)：12-16,5.DOI:10.3969/j.issn.1005-8982.2017.08.003
孕鼠铁缺乏对子代GH-IGF-1轴的影响
Effect of iron deficiency in pregnant rat on GH-IGF-1 axis of offspring
桑璐 1马雅静 1周祖钊 1王维 2林霖 1邱智枫1
作者信息
- 1. 石河子大学 医学院,新疆 石河子 832002
- 2. 江苏省涟水县人民医院,江苏 淮安 223400
- 折叠
摘要
Abstract
Objective To explore how iron deficiency in pregnant rats affected GH-IGF-1 axis of offspring and its mechanism. Methods Four-week-old healthy female Wistar rats were randomly divided into experimen-tal group ( =18) and control group ( =18). The rats of the experimental group were fed with low iron diet, while the control group were fed with normal diet. After four weeks, according to the 2 : 1 ratio, the rats were mated with healthy adult male rats. Hematological parameters of the rats' eye vein blood were detected, including hemoglobin (Hb), haematocrit (HCT), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC) and red blood cell distribution width (RDW). Pregnant rats were randomly drawn from each group to measure the iron of bone marrow. The survival rate and weight of newborn rat offspring were observed. The mRNA expressions of growth hormone (GH) in pituitary and insulin-like growth factor-1 (IGF-1) in liver of newborn rats were assessed by quantitative real-time PCR. Results The levels of Hb, HCT, MCV, MCH and MCHC in the experimental group were significantly lower ( <0.05) than those of the control group, while RDW increased remarkably ( <0.05). In the experimental group, iron in pregnant rats' bone marrow decreased significantly more than that in the control group, compared with which, the survival rate, weight, and the expression of GH mRNA and IGF- 1 mRNA of offspring decreased significantly ( < 0.05) in the experimental group. Conclusions Iron deficiency during pregnancy leads to low birth weight through down- regulating the expression of GH mRNA and IGF- 1 mRNA of offspring.关键词
铁缺乏/生长激素/胰岛素样生长因子-1Key words
iron deficiency/growth hormone/insulin-like growth factor-1分类
医药卫生引用本文复制引用
桑璐,马雅静,周祖钊,王维,林霖,邱智枫..孕鼠铁缺乏对子代GH-IGF-1轴的影响[J].中国现代医学杂志,2017,27(8):12-16,5.
