中国现代医学杂志2017，Vol.27Issue(8)：27-31,5.DOI:10.3969/j.issn.1005-8982.2017.08.006
阿司匹林诱生型脂氧素A4对小鼠脊髓撞击损伤后神经病理性疼痛的影响及机制探讨
Effect of ALT on neuropathic pain after spinal cord injury in mice
郭云翮 1李祥 1米卫东1
作者信息
- 1. 山西大同大学医学院 外科教研室,山西 大同 037009
- 折叠
摘要
Abstract
Objective To evaluate the effects of aspirin-triggered lipoxin A4 (ATL) on spinal neuroinflam-mation and neuropathic pain in mice model of spinal cord injury (SCI). Methods Modified Allen' hit model at T10 was carried out in adult FVB mice. To test if ATL can reduce neuroinflammation and neuropathic pain, each mouse received two intrathecal injections of ATL (300 pmol) or vehicle at 4 and 24 h after SCI. Sensi-tivity to mechanical stimulation of the hind paws was evaluated by von Frey monofilaments, and the neuro-inflammation was tested by measuring the mRNA expression levels of microglial markers and cytokines in the spinal cord samples after SCI. Also, microglia cultures prepared from mice cortical tissue were used to assess the direct effects of ATL on microglial activation and release of pro-inflammatory TNF-α. Results ATL treat-ment caused significant reductions in the intensity of mechanical pain hypersensitivity and spinal expression levels of microglial markers and pro-inflammatory cytokines induced by SCI, when compared to the control group. Notably, the increased expressions of the microglial marker IBA-1 and pro-inflammatory cytokine TNF-α were affected by the ATL treatment mostly. Conclusions Our results suggest that ATL can effectively modu-late microglial activation and TNF-α release through ALX receptors, ultimately reduce neuropathic pain in mice after SCI.关键词
阿司匹林诱生型脂氧素A4/脊髓损伤/神经病理性疼痛/小鼠Key words
aspirin-triggered lipoxin A4/spinal cord injury/neuropathic pain/mice分类
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郭云翮,李祥,米卫东..阿司匹林诱生型脂氧素A4对小鼠脊髓撞击损伤后神经病理性疼痛的影响及机制探讨[J].中国现代医学杂志,2017,27(8):27-31,5.
