中国畜牧杂志2017，Vol.53Issue(5)：37-41,5.DOI:10.19556/j.0258-7033.2017-05-037
焉耆马的DRA与DQA基因遗传多态性及其序列分析
Genetic Polymorphism and Sequence Analysis of DRA and DQA Gene in Yanqi Horse
摘要
Abstract
ELA-DRA*exon2 and ELA-DQA*exon2 of Yanqi horse were genotyped by polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) methods.Both the nucleotide sequences and the amino acid sequences of the different alleles were analyzed in these horses.The results showed four alleles of ELA-DRA*exon2 and nine alleles of ELA-DQA*exon2 were detected in 50 Yanqi horses,three new alleles were found by nucleotide sequences alignment.The estimation of the nucleotide sequences and the amino acid sequences of the different alleles in the ELA-DRA*exon2 and ELA-DQA*exon2 showed there was a higher ratio of non-synonymous substitutions in the antigen-binding site (ABS) region.The genetic polymorphism analysis showed that the PIC ofELA-DRA * exon2 is 0.4 in Yanqi horse,is moderately polymorphic.A Chi-square analysis suggested that the allele frequencies and genotype frequencies were in HardyWeinberg equilibrium in the Yanqi horse(x2 ＞x0.052).This paper would provide a theoretical basis for resistance breeding of horses.关键词
焉耆马/主要组织相容性复合体/DRA/DQA/PCR-SSCPKey words
Yanqi horse/MHC/DRA/DQA/PCR-SSCP分类
农业科技引用本文复制引用
于丽娟,张艳花,徐新明,吴伟伟,阿米妮古丽·阿不来孜,田可川..焉耆马的DRA与DQA基因遗传多态性及其序列分析[J].中国畜牧杂志,2017,53(5):37-41,5.基金项目
新疆维吾尔自治区公益性科研院所基本科研业务经费资助项目(KyGy2016004) （KyGy2016004）
新疆绒毛用羊遗传育种与繁殖重点实验室(XJYS1105) （XJYS1105）
