中国药理学通报2017，Vol.33Issue(5)：622-626,5.DOI:10.3969/j.issn.1001-1978.2017.05.007
前扣带回皮层中趋化因子CX3CL1对于大鼠慢性病理性疼痛的影响
Effects of chemokine on chronic neuropathic pain in anterior cingulate cortex
摘要
Abstract
Aim To investigate the effects of chemokine in the anterior cingulate cortex(ACC)of rats on the chronic neuropathic pain.Methods A model of trigeminal neuropathic pain was made by chronic constriction injury to the unilateral infraorbital nerve(CCI-ION).Eighty male Sprague-Dawley rats were randomly assigned into 4 groups(n=20): the sham group,the control group,the PBS treatment group and the anti-CX3CL1 treatment group.The unilateral infraorbital nerve was just exposed in the sham group; in the control group,the unilateral infraorbital nerve was exposed and ligated.The behavioral test was measured at 9 :00 am on day 1,3,5,7,14 after surgery separately.These rats were executed after behavioral tests,then tissues of ACC were removed to compare the protein expression levels of CX3CL1 and CD11b in two groups.The PBS solution and the CX3CL1 neutralizing antibody were injected into the ACC separately at 10 :00 am on the day with the highest level of CX3CL1 protein expression in the PBS treatment group and the anti-CX3CL1 treatment group.The behavioral test was measured 6 h later after the injection,and the tissue of ACC was removed to measure the protein expression levels of CX3CL1,CD11b and IL-1β of the two groups.Results At baseline,there was no significant difference in the feeling threshold among the four groups(P<005).Compared with the sham group,there was a significant reduction in the feeling threshold in the ipsilateral ION territory from 3 d to 14 d after CCI-ION in the control group(P<005).Rats given antibody in the anti-CX3CL1 treatment group showed an evident increase of the feeling threshold compared with the PBS group(P<005).Conclusion The ACC may take part in the chronic neuropathic pain via associating with the activated microglial cell and high-level of CX3CL1 expression.关键词
慢行病理性疼痛/眶下神经结扎/前扣带回皮层/小胶质细胞/趋化因子CX3CL1/CD11bKey words
chronic neuropathic pain/CCI-ION/ACC/microglial cell/CX3CL1/CD11b分类
医药卫生引用本文复制引用
支亦博,章洁,郭伟兵,陈春龙,李文君,刘清珍,刘健,李伟彦..前扣带回皮层中趋化因子CX3CL1对于大鼠慢性病理性疼痛的影响[J].中国药理学通报,2017,33(5):622-626,5.基金项目
江苏省自然科学基金资助项目(NoBK20131328) （NoBK20131328）
