中国药理学通报2017，Vol.33Issue(5)：627-629,3.DOI:10.3969/j.issn.1001-1978.2017.05.008
SM-1对人肝微粒体细胞色素P450亚型酶的抑制作用
Inhibitory effect of SM-1 on human liver microsomal cytochrome P450 enzyme
摘要
Abstract
Aim To investigate the effect of SM-1 on seven main cytochrome P450(CYP450)in human liver microsomes.Methods Substrate or SM-1 was incubated with human liver microsomes for 30 min in vitro,and divided into control group and experimental group.The effects of SM-1 on the main phase I metabolic enzymes in human liver microsomes was detected by HPLC.Phenacetin,bupropion,paclitaxel,tolbutamide,omeprazole,dextromethorphan,testosterone were investigated as probe drugs.Results Inhibition rate of SM-1 on the classical substrate of human liver microsomal CYP was 0.05％,3.37％,0.08％,2.07％,4.20％,-0.15％and 10.84％,respectively.Conclusions SM-1 may have inhibitory effect on CYP3A4.Attention should be paid to the interaction of clinical drug induced by CYP enzyme inhibition.关键词
SM-1/细胞色素P450/底物/抑制率/肝微粒体/亚型酶Key words
SM-1/cytochrome P450/substrate/inhibition rate/microsomes/subtypes of enzymes分类
医药卫生引用本文复制引用
陈燕芬,陈景秀,孙萌,丁俊垚,程泽能,胡高云,丁劲松,朱曲波..SM-1对人肝微粒体细胞色素P450亚型酶的抑制作用[J].中国药理学通报,2017,33(5):627-629,3.基金项目
国家科技部"重大新药创新与发展"重大专项项目(No2012ZX09103101-051) （No2012ZX09103101-051）
基础研究中国中央大学资助项目(No7601110179) （No7601110179）
中南大学研究生自主探索创新基金(No2016zzts494) （No2016zzts494）
