中国药理学通报2017，Vol.33Issue(5)：657-662,6.DOI:10.3969/j.issn.1001-1978.2017.05.014
MicroRNA-155在肝细胞癌对索拉非尼抗药中的作用研究
Effect of microRNA-155 on sorafenib resistance in hepatocellular carcinoma
摘要
Abstract
Aim To investigate the effect of microRNA-155(miR-155)on sorafenib resistance in hepatocellular carcinoma(HCC).Methods Lentivirus mediated miR-155 inhibition was transfected into SMMC-7721 cells,while lentivirus mediated miR-155 overexpression was transfected into HepG2 cells.The level of miR-155 was evaluated by qPCR.Cell viability and apoptosis were analyzed by cell counting kit-8(CCK-8)assay and flow cytometry,respectively.The protein expression of activated caspase-3 was measured by Western blot.Results Compared to control group,the expression of miR-155 was significantly downregulated in miR-155 inhibition lentivirus infected SMMC-7721 cells(P<0.01),sorafenib treatment markedly suppressed cell viability(P<0.05)and increased cell apoptosis(P<0.01),as well as enhanced the expression of activated caspase-3(P<0.01).However,HepG2 cells were infected by miR-155 overexpression lentivirus which deserved completely opposite results.Conclusion miR-155 may participate in sorafenib resistance in HCC and provide a promising molecular target for the treatment of HCC.关键词
微小RNA155/肝细胞癌/索拉非尼/抗药/慢病毒/活性caspase-3Key words
mircoRNA-155/hepatocellular carcinoma/sorafenib/drug resistance/lentivirus/activated caspase-3网络出版时间:2017-4-24 11:20分类
医药卫生引用本文复制引用
吕峰,王伟,艾麦提·牙森,金鑫,李德卫..MicroRNA-155在肝细胞癌对索拉非尼抗药中的作用研究[J].中国药理学通报,2017,33(5):657-662,6.基金项目
国家自然科学基金资助项目(No81470898) （No81470898）
