中国药理学通报2017，Vol.33Issue(5)：668-674,7.DOI:10.3969/j.issn.1001-1978.2017.05.016
胡黄连苷Ⅱ通过抑制cyto C/caspase-9/caspase-3通路发挥神经保护作用
Picroside Ⅱ plays a neuroprotective effect by inhibiting cyto C/caspase-9/caspase-3 signal pathway following ischemia/reperfusion injury in rats
摘要
Abstract
Aim To investigate the neuroprotective effect of picroside Ⅱ(PIC)on cyto C/caspase-9/caspase-3 signal pathway following ischemia/reperfusion(I/R)injury in rats.Methods Atractyloside(Atr)was selected as negative control,cyclosporin A(CsA)was selected as positive control,and PIC was selected as the treatment medicine.The I/R model was made by inserting a monofilament suture into internal carotid artery for 2 h,and then reperfused for 24 h.The cerebral infarction volume was detected by TTC staining,and the expression of cyto C,caspase-9 and caspase-3 were determined by immunohistochemical assay and Western blot.Results In model group,the cerebral infarct volume was obviously large;the expression of cyto C,caspase-9 and caspase-3 was increased significantly more than that in sham group(P<0.05).In PIC group,the cerebral infarct volume was significantly improved;the expression of cyto C,caspase-9 and caspase-3 was significantly decreased than that in model group(P<0.05).In Atr+PIC group,the rat infarction volume was reduced,and the expression of cyto C,caspase-9 and caspase-3 was significantly decreased than that in Atr group(P<0.05).Conclusion The mechanism of PIC inhibiting neuron apoptosis in focal cerebral I/R rats might be through down-regulating the expression of cyto C,caspase-9 and caspase-3.关键词
胡黄连苷Ⅱ/脑/缺血/再灌注损伤/cytoC/caspase-9/caspase-3信号通路/神经保护/大鼠Key words
picroside Ⅱ/cerebrum/ischemic/reperfusion injury/cyto C/caspase-9/caspase-3 signal pathway/neuroprotection/rats分类
医药卫生引用本文复制引用
张红艳,翟丽,王婷婷,李珊,张艳辉,郭云良..胡黄连苷Ⅱ通过抑制cyto C/caspase-9/caspase-3通路发挥神经保护作用[J].中国药理学通报,2017,33(5):668-674,7.基金项目
国家自然科学基金资助项目(No81274116) （No81274116）
