中国药理学通报2017，Vol.33Issue(5)：685-691,7.DOI:10.3969/j.issn.1001-1978.2017.05.019
杜鹃花总黄酮对缺血/再灌注损伤模型大鼠脑基底动脉TRPV4的作用研究
Effects of total flavones of rhododendra on transient receptorpotential vanilloid receptor 4 in cerebral basilar arteries ofrats subjected to ischemia/reperfusion injury
摘要
Abstract
Aim To research the effects of total flavones of rhododendra(TFR)on transient receptor potential vanilloid receptor 4(TRPV4)in cerebral basilar arteries(CBA)of rats subjected to ischemia/reperfusion(IR)injury.Methods The model of total brain IR was established by four-artery occlusion(4-VO)method in rats.Arterial pressure perfusion and cell membrane potential recording methods were used for surveying the dilatation and hyperpolarization of TFR and ruthenium red(RR,an inhibitor of TRPV4)in the KCl-preconstricted CBA ex vivo in rats subjected to IR.Quantitative real-time polymerase chain reaction(qRT-PCR)and Western blot were utilized to investigate the TRPV4 mRNA and protein expressions of TFR and RR in cerebrovascular endothelial cells of CBA in vivo in rats subjected to IR.Results 11～2 700 mg·L-1 TFR significantly induced concentration-dependent hyperpolarization and dilatation in the KCl-preconstricted CBA in rats subjected to IR.TFR still produced degenerative hyperpolarization and dilatation by removal of endothelium in CBA,which was remarkably attenuated as compared with endothelium-intact group(P<0.01).After removal of NO and PGI2 vasodilatation,TFR obviously elicited the hyperpolarization and dilatation that were further decreased by RR(an inhibitor of TRPV4)in IR CBAs.TFR pretreatment apparently increased the level of TRPV4 mRNA and protein expressions in IR CBAs.These effects were restrained by RR,an inhibitor of TRPV4.Conclusions TFR could mediate endothelium-dependent and endothelium-independent effects.The endothelium-derived dilatation may be related to the increase of endothelium activity and endothelium-derived hyperpolarizing factor(EDHF)generation and release that have been promoted by TFR,and secondarily activating TRPV4,which results in Ca2+ inflow and subsequent hyperpolarization of vascular smooth muscle cell membrane and vasorelaxation.关键词
杜鹃花总黄酮/缺血/再灌注损伤/脑基底动脉/超极化作用/血管舒张效应/内皮衍生性超极化因子/瞬时感受器电位通道香草酸受体亚型ⅣKey words
TFR/ischemia/reperfusion injury/cerebral basilar artery/hyperpolarization/vasodilatation/endothelium-derived hyperpolarizing factor/TRPV4分类
医药卫生引用本文复制引用
韩军,程小龙,胡坤媚,徐杭杭,陈志武..杜鹃花总黄酮对缺血/再灌注损伤模型大鼠脑基底动脉TRPV4的作用研究[J].中国药理学通报,2017,33(5):685-691,7.基金项目
国家自然科学基金资助项目(No81173596) （No81173596）
安徽省教育厅自然科学基金重点项目(NoKJ2015A157) （NoKJ2015A157）
