中国药理学通报2017，Vol.33Issue(5)：729-732,4.DOI:10.3969/j.issn.1001-1978.2017.05.026
基于TLR4-NF-κB的柴胡黄芩水煎液抑制CCl4诱导大鼠肝星状细胞激活作用机制研究
Inhibitory effects of Bupleurum chinense-Scutellaria baicalensisdecoction on activation of rat HSC induced by CCl4
摘要
Abstract
Aim To investigate the inhibitory effect of Bupleurum chinense-Scutellaria baicalensis decoction(CQ)on activation of rat HSC induced by CCl4 and the mechanism.Methods Cells in logarithmic growth phase were cultured in culture medium without FBS for 24 h.After disassociated using 0.25％EDTA-trypsin,the cells were seeded into respective plates at the density of 1.5×109· L-1 and cultured overnight.The cells were divided into the following groups:control group(no treatment),model group(treated with 6 mmol·L-1 CCl4 for 24 h),CQ groups(pretreated with 6 mmol·L-1 CCl4 for 24 h and 400,500,600 mg·L-1 CQ for 24 h).Concentrations of hyaluronidase(HA),laminin(LN),procollagen Ⅲ(PCⅢ),collagen type Ⅳ(Ⅳ-C)were assayed by ELISA kits.Gene expressions of Toll-like receptor 4(TLR4)and NF-κB were examined by RT-PCR analysis.Protein expression of TLR4 and NF-κB was examined by Western blot.Results CQ could significantly inhibit cell proliferation induced by CCl4.Furthermore,CQ at 600 mg·L-1 significantly downregulated gene and protein expressions of TLR4 and NF-κB.And CQ reduced the secretion of HA,LN,PCⅢ,Ⅳ-C.Further studies disclosed that the TLR4 inhibitor TAK-242 and NF-κB inhibitor BAY 11-7082 could significantly inhibit the gene and protein expressions of NF-κB,but could not change gene and protein expression of TLR4,and reduced the secretion of HA,LN,PCⅢ,Ⅳ-C.Conclusion CQ could inhibit inflammatory responses in HSC induced by CCl4 probably by inhibiting the transcription activity and protein expression of TLR4-NF-κB,which indicates its possible therapeutic effect on liver fibrosis.关键词
肝纤维化/柴胡-黄芩水煎液/HSC/CCl4诱导/TLR4/NF-κBKey words
liver fibrosis/Bupleurum chinense-Scutellaria baicalensis decoction/HSC/induced by CCl4/TLR4/NF-κB分类
医药卫生引用本文复制引用
李敏,韩艳,王华林,王義雯,李静,王斌..基于TLR4-NF-κB的柴胡黄芩水煎液抑制CCl4诱导大鼠肝星状细胞激活作用机制研究[J].中国药理学通报,2017,33(5):729-732,4.基金项目
陕西省科技厅项目(No2013jk4023) （No2013jk4023）
陕西省中医药管理局项目(Nozy06) （Nozy06）
陕西省教育厅项目(No12JK1016) （No12JK1016）
