中山大学学报（医学科学版）2017，Vol.38Issue(3)：372-378,7.
急性坏死性胰腺炎合并糖尿病大鼠早期细菌移位的研究
Research of Acute Necrotizing Pancreatitis Associated with Diabetes Mellitus on Early Bacterial Translocation in Rat
摘要
Abstract
[Objective] To explore whether diabetes mellitus (DM) can influence the early bacterial translocation (BT) and progression of acute necrotizing pancreatitis (ANP) for guiding the early clinical treatment.[Methods] 35 Wistar male rats were randomly allocated to 4 groups,Group ANP associatcd with DM (DM+ANP,n =10):DM underwent induction of ANP;Group DM (n =10):DM underwent laparotomy with only manipulation of the pancreas and duodenum;Group ANP (n =10):non-DM underwent induction of ANP;Group sham operation (SO,n =5):non-DM underwent SO.After 12 h of the induction of ANP or laparotomy,the following parameters were analyzed:bacterial culture and identification of portal vein blood,mesenteric lymph nodes (MLNs),pancreas and liver,and calculate the total incidence of BT;serum amylase and endotoxin levels of portal vein blood;histological assessment of pancreas and ileum lesions.[Results] All animals except 3 in group DM+ANP (mortality rates:30％) and 1 in group ANP (mortality rates:10％) survived the experiment.The total incidence of BT was 23/28 (82.1％) in group DM+ANP whereas 16/36 (44.4％) in group ANP (P =0.002).Gram-positive bacteria were 17/23 (73.9％),3/16 (18.8％) in group DM+ANP and group ANP,respectively (P =0.001).Amylase activity (2302 ± 346) U/L in group ANP increased significantly (P =0.000) compared with other groups.However,group DM+ANP (501 ± 142) U/L decreased significantly (P =0.001) in comparison to group SO.Regarding to endotoxin concentrations and the severity of pancreas and ileum lesions,group DM + ANP increased significantly compared with group ANP,group DM and group SO (P ＜ 0.05).[Conclusion] Gram-positive bacteria translocates more frequently than Gram-negative bacteria in the early period of DM+ANP rats.DM aggravates the progression of ANP and increases early bacterial translocation,endotoxemia and severity of pancreas and ileum lesions.关键词
急性坏死性胰腺炎/糖尿病/细菌移位/内毒素血症/链脲佐菌素Key words
acute necrotizing pancreatitis/diabetes mellitus/bacterial translocation/endotoxemia/streptozotocin分类
医药卫生引用本文复制引用
伍先权,苏永辉,卜巨源,李霄凌,侯鸿发,侯冰宗..急性坏死性胰腺炎合并糖尿病大鼠早期细菌移位的研究[J].中山大学学报（医学科学版）,2017,38(3):372-378,7.基金项目
广东省医学科学技术研究基金(B2013156) （B2013156）
