中国中西医结合杂志2017，Vol.37Issue(5)：552-556,5.DOI:10.7661/j.cjim.20170315.039
不同归经补益中药对骨转移癌CXCR4表达的影响
Effects of Different Channel Tropism Tonic Herbs on CXCR4 Expression in Bone Metastasis Cancer
摘要
Abstract
Objective To observe inhibition and mechanisms of different channel tropism tonic herbs on CXCR4 expression.Methods Bone metastatic carcinoma model was established according to Medhurst method with metastatic breast cancer cells in rats.Forty rats were stratified by body weight.Then,the rats were divided into 5 groups according to random digit table,i.e.,the sham-operated group,the model group,Shen channel group (Fructus Psoraleae,Cistanche deserticola,Fructus Alpiniae oxyphyllae),Fei channel group (Codonopsis pilosula,Astragalus mongholicus,Lilium brownii),and Gan channel group (Bear Bile Powder),8 in each group.After modeling rats in Shen channel group,Fei channel group,and Gan channel group were administered with corresponding decoctions (10 mL · kg-1 · d-1) by gastrogavage (equivalent to 10 times that 45 g · kg-1 · d-1 crude drugs).Equal volume of deionized water was administered to rats in the sham-operated group and the model group by gastrogavage.All medications lasted for 20 days.Effects of different channel tropism tonic herbs were observed.Imaging methods were applied to score and measure the tumor volume,and morphological changes were observed by HE staining.In addition,expressions of CXC chemokine receptors (CXCR4) were semi-quantitatively detected using immunohistochemistry.Results In bone metastatic carcinoma model bone destruction and tumor growth occurred.Compared with the sham-operated group,tumor volume were increased significantly (P ＜0.01),bone destruction were severer and scores increased (P ＜0.01),as well as CXCR4 expression levels were significantly higher in the model group (P ＜0.01).Compared with the model group,bone destruction was obviously attenuated,and scores decreased (P ＜0.05),tumor volume was reduced (P ＜0.05),and CXCR4 expression level was obviously lowered (P ＜0.01) in Shen channel group.However,there was no significant difference in aforesaid parameters between Fei channel group and Gan channel group (P ＞0.05).Conclusions Shen channel tropism tonic herbs had certain inhibition on bone metastatic cancer foci.It might fight against oriental proliferation and invasion possibly by blocking SDF-1/CXCR4 biological axis and downstream signaling pathways.Fei and Gan channel tropism tonic formulas had no similar effects.Different channel tropism tonic formulas had specific targeting functions.关键词
骨趋化因子受体4/补益中药/归经Key words
CXC chemokine receptor4/channel tropism tonic formulas/channel tropism引用本文复制引用
林荔钦,朱世杰..不同归经补益中药对骨转移癌CXCR4表达的影响[J].中国中西医结合杂志,2017,37(5):552-556,5.基金项目
国家自然科学基金资助项目(No.81173374) （No.81173374）
