中国中西医结合杂志2017，Vol.37Issue(5)：579-583,5.DOI:10.7661/j.cjim.20170315.044
痰瘀同治方调控PPARγ/NF-κB通路对大鼠动脉粥样硬化斑块内血管新生的影响
Effect of Tanyu Tongzhi Recipe on Angiogenesis In Atherosclerosis Plaque Rats by Regulating PPARγ/NF-κB Pathways
摘要
Abstract
Objective To observe the inhibitory effect of Tanyu Tongzhi Recipe (TYTZR) on plaque angiogenesis,and to observe the regulatory mechanism of PPARγ/NF-κB pathways in its process.Methods Fifty Wistar rats were randomly divided into 5 groups,i.e.,the blank control group,the model group,the rosiglitazone group,the low dose TYTZR group,the high dose TYTZR group,10 in each group.Rats in the blank control group were fed with basic forage,while those in the rest 4 groups were fed with high fat +vitamin D3 overload + balloon injury of arterial intima to establish atherosclerosis (AS) rat model.Rats in the blank control and the model group were administered with normal saline (4 mL) by gastrogavage.Rats in the rosiglitazone group were fed with rosiglitazone at the daily dose of 3 mg/kg by gastrogavage.Rats in low and high dose TYTZR groups were fed with TYTZR crude drug at the daily dose of 7.5 and 30.0 g/kg respectively by gastrogavage.All treatment lasted for 6 successive weeks.Blood lipids (TC,TG,LDL-C) and inflammatory factors [C-reactive protein (CRP),IL-1,monocyte chemotactic protein-1 (MCP-1)] were measured.The density of neovessels was observed by immunohistochemical staining CD31.The protein expression levels of PPARγand NF-κB in aorta were detected by Western blot.Correlation analyses were performed between protein expression levels of PPARγ and NF-κB in each group.Results (1) Compared with the blank control group,the levels of TC,CRP,IL-1,and MCP-1 significantly increased in model group (P ＜0.05).Compared with the model group,the levels of TC and LDL-C significantly decreased in the high dose TYTZR group (P ＜0.05);the levels of TC and TG significantly decreased in the rosiglitazone group (P ＜0.05);expression levels of CRP,IL-1,and MCP-1 significantly decreased in the high dose TYTZR group and the rosiglitazone group (P ＜0.05).(2) CD31 immunohistochemical staining showed that the expression of angiogenesis was the richest in the model group,as compared with the blank control group (P ＜0.05).Compared with the model group,the density of neovessels in plagues decreased significantly in each intervention group (P ＜0.05).(3) Compared with the blank control group,the expression levels of PPARγ protein decreased (P ＜0.05) and the level of NF-κB protein increased (P ＜ 0.05) in the model group.Compared with the model group,the protein expression level of PPARγincreased and the protein expression of NF-κB decreased in the rosiglitazone group,high and low dose TYTZR groups (P ＜0.05).Negative correlation existed between the protein expression level of PPARγand the protein expression level of NF-κB (r =-0.635,P ＜0.05).Conclusions TYTZR had the roles of lipid lowering,anti-inflammatory,and antiangiogenic effect.The activation of PPARγ/NF-κB pathways might be one of the mechanisms of TYTZR for inhibiting plaque angiogenesis.关键词
痰瘀同治方/动脉粥样硬化/PPARγ/NF-κB通路/新生血管Key words
Tanyu Tongzhi Recipe/atherosclerosis/PPARγ/NF-κB pathway/angiogenesis引用本文复制引用
蔡宏文,缪静,周鑫斌,武丽,庄钦,毛威..痰瘀同治方调控PPARγ/NF-κB通路对大鼠动脉粥样硬化斑块内血管新生的影响[J].中国中西医结合杂志,2017,37(5):579-583,5.基金项目
浙江省中医药科研基金项目(No.2013ZA054,No.2016ZA073) （No.2013ZA054,No.2016ZA073）
浙江省医药卫生研究计划(No.2013KYB186) （No.2013KYB186）
