长春中医药大学学报2017，Vol.33Issue(3)：358-361,4.DOI:10.13463/j.cnki.cczyy.2017.03.005
电项针对失眠大鼠脑干单胺类神经递质含量的影响
Research of electro-nape-acupuncture on the contents of monoamine neurotransmitters in brainstem of insomnia rats
摘要
Abstract
Objective To observe the effect of different frequencies of electro-nape-acupuncture (ENA) on the contents of monoamine neurotransmitters 5-hydroxytryptamine (5-HT), dopamin (DA) and norepinephrine (NE) in brainstem of insomnia rats induced by PCPA, to discuss primarily the mechanism of promoting sleep and therapeutic efficacy of different frequencies of ENA. Methods Fifty Wistar rats were randomly divided into five groups, those are normal group, model group, ENA group Ⅰ (stimulus frequency of 2 Hz), ENA group Ⅱ (stimulus frequency of 50 Hz) and ENA group Ⅲ (stimulus frequency of 100 Hz). The animal models of insomnia were established by para-chlorophenylalanine (PCPA) intraperitoneal injection in each group except normal group. After duplicating the models successfully, each ENA group rats were treated by ENA therapy at different stimulus frequencies respectively for seven consecutive days, 1 time/day, 30min/time. After treatment the brainstems were taken and measured the contents of neurotransmitters 5-HT, DA and NE. Results The content of 5-HT were significantly lower and the contents of DA and NE were higher in the animal model of rats than that in the normal group, there were statistically significant differences (P<0.05); After the seven days of ENA treatment, the 5-HT content increased and the DA and NE contents decreased in brainstems of insomnia rats. Compared with the model group, there were significant differences (P<0.05). Among the ENA groups, ENA group Ⅰ was greatly superior to ENA group Ⅱ and ENA group Ⅲ at regulating the levels of monoamine neurotransmitters (P<0.05), however, there were no statistically significant difference between ENA group Ⅱ and ENA group Ⅲ (P >0.05). Conclusion The mechanism of ENA improve the structure of sleep may involve the regulation of monoamine neurotransmitters 5-HT, DA and NE contents in brainstem of insomnia rats.关键词
失眠/电项针/脑干/单胺类神经递质Key words
insomnia/electro-nape-acupuncture/brainstem/monoamine neurotransmitter分类
医药卫生引用本文复制引用
吴建丽,王天琪,王永亮,杜冬梅,史文强,王薇..电项针对失眠大鼠脑干单胺类神经递质含量的影响[J].长春中医药大学学报,2017,33(3):358-361,4.基金项目
哈尔滨市科技局科技创新人才研究专项资金"基于电项针对睡眠剥夺大鼠促眠作用的机制研究"(2015RAQXJ093). （2015RAQXJ093）
