福建农林大学学报（自然科学版）2017，Vol.46Issue(3)：305-310,6.DOI:10.13323/j.cnki.j.fafu(nat.sci.).2017.03.012
莆田黑猪不同组织DNA甲基化的MSAP分析
DNA methylation detection on different tissues of Putian black swine based on MSAP
摘要
Abstract
Methylation sensitive amplification polymorphism ( MSAP ) method was used to detect methylation levels in various organs of Purain black swine at day 1, 25, and 100. The results showed that methylation levels of heart and liver tended to decrease with age, and varied significantly among different growth stages ( P<0.05) . Although methylation levels were not different between day 1 and 25 ( P>0.05) , methylation level at day 25 was significantly higher than that at day 210( P<0.05) . Methylation levels of fat tis-sue and ear at day 25 were significantly higher than those at day 1 and 210 (P<0.05). Tail methylation level did not vary signifi-cantly among different growth stages ( P>0.05) . Furthermore, genomic DNA methylation levels varied significantly between liver and fat, and between liver and ear at 3 growth stages ( P<0.05) , indicating that tissue specificity of DNA methylation existed between liver and fat, and liver and ear tissues, respectively.关键词
DNA甲基化/组织/甲基化敏感扩增多态性(MSAP)/莆田黑猪Key words
DNA methylation/tissue/methylation sensitive amplification polymorphism(MSAP)/Putian black swine分类
农业科技引用本文复制引用
胡宇平,张金耀,林瑞意,林威敏,陈静,汪稳,肖天放..莆田黑猪不同组织DNA甲基化的MSAP分析[J].福建农林大学学报（自然科学版）,2017,46(3):305-310,6.基金项目
科技部科技基础性工作专项(2014FY120800) （2014FY120800）
福建省现代农业(生猪)产业技术体系建设项目(2014-357). （生猪）
