南方农业学报2017，Vol.48Issue(5)：800-805,6.DOI:10.3969/j.issn.2095-1191.2017.05.007
接种AM真菌对采煤沉陷地复垦植物光合作用和抗逆性的影响
Effects of Arbuscular mycorrhizal fungus on photosynthesis and stress resistance of reclamation plants in coal mining subsidence areas
摘要
Abstract
[Objective]The experiment was conducted to investigate the effects of Arbuscular mycorrhizal fungus on growth, photosynthesis and stress resistance of reclamation plants in coal mining subsidence areas, and provide some theo-retical references and suggestions for ecological restoring of mining areas. [Method]Four reclamation plant species including Prunus discadenia, P. dictyneura, Xanthoceras sorbifolia and Armeniaca sibirica were planted in Shendong coal mining subsidence area in Inner Mongolia. For each plant, inoculation with A. mycorrhizal fungus and no inoculation control(CK) treatments were set. Effects of A. mycorrhizal fungus on plant height, ground diameter, mycorrhiza colonization rate, root activity, net photosynthesis rate(Pn), stomatal conductance(Gs), intercellular CO2 concentration(Ci),transpiration rate(Tr), relative water content, soluble sugar content, proline content, relative electric conductivity and catalase activity were de-tected, and the relations among them were analyzed. [Result]Compared with CK, P. discadenia, P. dictyneura, X. sorbifo-lia and A. sibirica formed typical mycorrhizal structure with A. mycorrhizal fungus after inoculation. The mycorrhizal colo-nization rates were 65.42%-76.22%, significantly higher than the corresponding CK(P<0.05, the same below). Root activity increased by 17.3%(P. discadenia), 23.0%(P. dictyneura), 32.5%(X. sorbifolia) and 28.7%(A. sibirica). In treat-ment groups, Pn, Gs, Tr, Ci, relative water content, soluble sugar content, proline content and catalase activity significant-ly increased, which promoted resistance to drought and high temperature of reclamation plants. Correlation analysis demon-strated that mycorrhiza colonization rate was significantly positively correlated with root activity , Pn, Gs, Tr, relative water content, soluble sugar, proline and catalase activity, whereas it was significantly negatively correlated with relative electric conductivity and Ci. [Conclusion]A. mycorrhizal fungus inoculation is beneficial for reclamation plants in Shendong coal mining subsidence area. Therefore, A. mycorrhizal fungus can be promoted in coal mining subsidence areas with similar conditions as Shendong coal mining area.关键词
AM真菌/采煤沉陷地/复垦植物/光合作用/抗逆性/相关性Key words
Arbuscular mycorrhizal fungus/coal mining subsidence area/reclamation plant/photosynthesis/stress resistance/correlation分类
农业科技引用本文复制引用
王志刚,毕银丽,李强,裘浪,江彬,刘浩..接种AM真菌对采煤沉陷地复垦植物光合作用和抗逆性的影响[J].南方农业学报,2017,48(5):800-805,6.基金项目
国家自然科学基金项目(51574253) （51574253）
国家高技术研究发展计划项目(2013AA102904) （2013AA102904）
神华科技创新项目(CSIE13023514) （CSIE13023514）
